[提取物名称]水提取物对溴苯诱导的大鼠肝脏胞质谷胱甘肽S-转移酶同工酶变化的保护作用。 （注：原文中“of ”后面内容缺失）
Protective effect of aqueous extract of against bromobenzene induced changes on cytosolic glutathione S-transferase isozymes in rat liver.
作者信息
Gopi Sriram, Vadde Ramakrishna, Setty Oruganti H
机构信息
Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.
Department of Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa 516003, India.
出版信息
Biochem Biophys Rep. 2017 Apr 6;10:208-214. doi: 10.1016/j.bbrep.2017.04.001. eCollection 2017 Jul.
The aim of this study was to investigate beneficial effect of aqueous extract of (AEPF) on bromobenzene (BB) induced changes on cytosolic glutathione S-transferase (GST) isozymes in rat liver. Administration of BB significantly decreased the activity of GST, however, prior administration of AEPF prevented the BB induced decrease in GST activity. Further the cytosolic GSTs were purified from 3 groups of animals (control, BB and AEPF+BB administered) and resolved into three protein bands on SDS-PAGE. Densitometric analysis showed a significant decrease in BB group compared to control. Further, 2D PAGE analysis resolved these proteins into 8 bands which were identified as five isozymes of alpha, two of Mu and one of theta by MALDI-TOF MS and also observed decreased levels of isozymes in BB group. However, on prior administration of AEPF significantly prevented the BB induced decrease in GSTs and restored to normal levels.
本研究的目的是探讨（AEPF）水提取物对溴苯（BB）诱导的大鼠肝脏胞质谷胱甘肽S-转移酶（GST）同工酶变化的有益作用。给予BB显著降低了GST的活性，然而，预先给予AEPF可防止BB诱导的GST活性降低。此外，从3组动物（对照组、BB组和给予AEPF + BB组）中纯化胞质GST，并在SDS-PAGE上分离为三条蛋白带。光密度分析显示，与对照组相比，BB组显著降低。此外，二维聚丙烯酰胺凝胶电泳分析将这些蛋白质分离为8条带，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定为α的五种同工酶、Mu的两种同工酶和θ的一种同工酶，并且还观察到BB组同工酶水平降低。然而，预先给予AEPF可显著防止BB诱导的GST降低并恢复到正常水平。
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