α-酮戊二酸可保护细胞免受可见光诱导的光毒性作用。

Xu Feng, Wang Jin, Zhao Guo-Ping

CAS Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.

State Key Laboratory of Genetic Engineering, Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200433, China.

Biochem Biophys Rep. 2016 Nov 10;9:22-28. doi: 10.1016/j.bbrep.2016.11.002. eCollection 2017 Mar.

It has been known that some species, including the model strain , are vulnerable to visible light. Much evidence demonstrated that the phototoxicity induced by visible light is a consequence of the formation of intracellular reactive oxygen species (ROS), which are potentially harmful to cells. In this study, we found that α-ketoglutarate (α-KG) has a protective role against the phototoxicity in . It could be because that α-KG can detoxify the ROS with the concomitant formation of succinate, which mediates the cells getting into anaerobiosis to produce more NADH and maintain intracellular redox homeostasis, a situation that was demonstrated by overexpressing in . This finding, therefore, connects the central metabolites with the bacterial resistance against phototoxicity effect induced by visible light.

已知包括模式菌株在内的一些物种对可见光敏感。大量证据表明，可见光诱导的光毒性是细胞内活性氧（ROS）形成的结果，ROS对细胞具有潜在危害。在本研究中，我们发现α-酮戊二酸（α-KG）对[具体物种]的光毒性具有保护作用。这可能是因为α-KG可以通过伴随琥珀酸的形成来清除ROS，介导细胞进入厌氧状态以产生更多的NADH并维持细胞内氧化还原稳态，这一情况在[具体物种]中过表达[相关基因]时得到了证实。因此，这一发现将中心代谢物与细菌对可见光诱导的光毒性效应的抗性联系了起来。