具有I.T错配碱基对的八核苷酸双链体的精细晶体结构。
Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs.
作者信息
Cruse W B, Aymani J, Kennard O, Brown T, Jack A G, Leonard G A
机构信息
University Chemical Laboratory, Cambridge, UK.
出版信息
Nucleic Acids Res. 1989 Jan 11;17(1):55-72. doi: 10.1093/nar/17.1.55.
Abstract
The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7A. The sequence crystallises in space group P6(1), with unit cell dimensions a = b = 45.07, c = 45.49A. The refinement converged with a crystallographic residual R = 0.14 and the location of 81 solvent molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C) base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure shows it to be essentially isomorphous with that reported for d(GGGGCTCC) with the mispairs adopting a "wobble" conformation. Conformational parameters and base stacking interactions are compared to those for the native duplex d(GGGGCCCC) and other similar sequences. A rationale for the apparent increased crystal packing efficiency and lattice stability of the I.T octamer is given.
摘要
已通过单晶X射线衍射技术确定了合成脱氧八聚体d(GGIGCTCC)的结构，分辨率为1.7埃。该序列在空间群P6(1)中结晶，晶胞尺寸a = b = 45.07，c = 45.49埃。精修收敛于晶体学残余R = 0.14以及81个溶剂分子的位置。该八聚体形成一个具有6个沃森-克里克（G.C）碱基对和2个肌苷-胸腺嘧啶（I.T）对的A-DNA双链体。结构精修表明它与报道的d(GGGGCTCC)基本同晶型，错配采用“摆动”构象。将构象参数和碱基堆积相互作用与天然双链体d(GGGGCCCC)及其他相似序列进行了比较。给出了I.T八聚体明显提高的晶体堆积效率和晶格稳定性的原理。
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