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寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。

Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.

作者信息

Gait M J, Matthes H W, Singh M, Sproat B S, Titmas R C

出版信息

Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.

DOI:10.1093/nar/10.20.6243
PMID:7177846
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326914/
Abstract

A new kieselguhr-polydimethylacrylamide support has been used in a continuous flow, column system for solid phase synthesis of oligodeoxyribonucleotides by a phosphotriester procedure. Using only protected mononucleotides a 14-mer, 20-mer and 27-mer were assembled in high repetitive yield using a simple manually operated, bench top apparatus.

摘要

一种新型的硅藻土-聚二甲基丙烯酰胺载体已用于连续流动柱系统，通过磷酸三酯法固相合成寡脱氧核糖核苷酸。仅使用受保护的单核苷酸，使用简单的手动台式仪器以高重复产率组装了14聚体、20聚体和27聚体。

相似文献

1
Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.寡脱氧核糖核苷酸的快速合成。VII. 在硅藻土-聚酰胺载体上通过连续流动磷酸三酯法固相合成寡脱氧核糖核苷酸。
Nucleic Acids Res. 1982 Oct 25;10(20):6243-54. doi: 10.1093/nar/10.20.6243.
2
Comparison of controlled pore glass and Kieselguhr-polydimethylacrylamide composite as supports for solid-phase synthesis of 23-residue oligodeoxyribonucleotides in milligram amounts.
Anal Biochem. 1985 May 15;147(1):63-74. doi: 10.1016/0003-2697(85)90009-0.
3
Solid phase phosphotriester synthesis of large oligodeoxyribonucleotides on a polyamide support.在聚酰胺载体上固相磷三酯法合成大型寡脱氧核糖核苷酸。
Nucleic Acids Res. 1980 Nov 25;8(22):5193-205. doi: 10.1093/nar/8.22.5193.
4
Continuous-flow phosphotriester method for solid-phase synthesis of oligodeoxyribonucleotides.用于寡脱氧核糖核苷酸固相合成的连续流动磷酸三酯法。
Cold Spring Harb Symp Quant Biol. 1983;47 Pt 1:393-401. doi: 10.1101/sqb.1983.047.01.046.
5
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
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Rapid synthesis of oligodeoxyribonucleotides. V. Further studies in solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡聚脱氧核糖核苷酸的快速合成。V. 通过磷酸三酯中间体进行寡聚脱氧核糖核苷酸固相合成的进一步研究。
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Silica gel: an improved support for the solid-phase phosphotriester synthesis of oligonucleotides.硅胶：用于寡核苷酸固相磷酸三酯合成的一种改进载体。
Nucleic Acids Res. 1982 Nov 25;10(22):7439-48. doi: 10.1093/nar/10.22.7439.
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Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method.多核苷酸的固相合成。VIII. 用磷酸三酯固相法合成混合寡脱氧核糖核苷酸。
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A rapid deprotection procedure for phosphotriester DNA synthesis.一种用于磷酸三酯DNA合成的快速脱保护程序。
Nucleic Acids Res. 1984 Sep 11;12(17):6853-9. doi: 10.1093/nar/12.17.6853.

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EMBO J. 1983;2(11):1893-900. doi: 10.1002/j.1460-2075.1983.tb01676.x.
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Filter disc supported oligonucleotide synthesis by the phosphite method.滤膜支持的亚磷酸酯法寡核苷酸合成。
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本文引用的文献

1
Solid-phase synthesis of polynucleotides. III. Synthesis of polynucleotides with defined sequences by the block coupling phosphotriester method.多核苷酸的固相合成。III. 用片段偶联磷酸三酯法合成具有特定序列的多核苷酸。
Nucleic Acids Res. 1980 Nov 25;8(22):5491-505. doi: 10.1093/nar/8.22.5491.
2
Rapid synthesis of oligodeoxyribonucleotides. IV. Improved solid phase synthesis of oligodeoxyribonucleotides through phosphotriester intermediates.寡脱氧核糖核苷酸的快速合成。IV. 通过磷酸三酯中间体改进寡脱氧核糖核苷酸的固相合成
Nucleic Acids Res. 1980 Mar 11;8(5):1081-96. doi: 10.1093/nar/8.5.1081.
3
Solid-phase synthesis of polynucleotides: V. Synthesis of oligodeoxyribonucleotides by the phosphomonotriazolide method.多核苷酸的固相合成：V. 用磷酸单三唑酯法合成寡脱氧核糖核苷酸
Nucleic Acids Symp Ser. 1980(7):281-91.
4
Solid phase synthesis of oligonucleotides on a crosslinked polyacrylmorpholide support.在交联聚丙烯酰吗啉载体上进行寡核苷酸的固相合成。
Nucleic Acids Symp Ser. 1980(7):233-41.
5
Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
Nucleic Acids Res. 1981 Apr 10;9(7):1691-706. doi: 10.1093/nar/9.7.1691.
6
Syringe method for stepwise chemical synthesis of oligonucleotides.用于寡核苷酸逐步化学合成的注射器法
Nucleic Acids Res. 1982 May 25;10(10):3249-60. doi: 10.1093/nar/10.10.3249.
7
Solid phase synthesis of polynucleotides. VI. Further studies on polystyrene copolymers for the solid support.多核苷酸的固相合成。VI. 关于用于固相载体的聚苯乙烯共聚物的进一步研究。
Nucleic Acids Res. 1982 Mar 11;10(5):1755-69. doi: 10.1093/nar/10.5.1755.
8
Synthesis of oligodeoxyribonucleotides on silica gel support.硅胶载体上寡脱氧核糖核苷酸的合成。
Nucleic Acids Res. 1981 Jun 25;9(12):2807-17. doi: 10.1093/nar/9.12.2807.
9
Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).寡核苷酸在纤维素上的合成。第二部分：编码胃抑制多肽（GIP）的22个寡脱氧核苷酸的合成设计与合成策略。
Nucleic Acids Symp Ser. 1980(7):225-32.
10
Rapid synthesis of oligodeoxyribonucleotides. III. Effect of added carboxylate ion on the efficiency of internucleotide bond formation. Solid-phase synthesis of the dodecanucleotide, d(pT-A-A-C-T-G-C-T-C-A-C-T).
Nucleic Acids Res. 1979 Apr;6(4):1259-68. doi: 10.1093/nar/6.4.1259.