Takahashi Y, Kato K, Hayashizaki Y, Wakabayashi T, Ohtsuka E, Matsuki S, Ikehara M, Matsubara K
Proc Natl Acad Sci U S A. 1985 Apr;82(7):1931-5. doi: 10.1073/pnas.82.7.1931.
A synthetic DNA based on the known amino acid sequence of the brain/gut peptide cholecystokinin (CCK) was synthesized. This DNA contained deoxyinosines at ambiguous codon positions and was used as a probe to isolate the CCK gene directly from a human genomic library. Nucleotide sequence analysis of the isolated gene revealed that human preprocholecystokinin consists of 115 amino acid residues, with 11 amino acids in common with the human gastrin precursor, another member of the gastrin-CCK family, and that the coding region is separated by a single, long intron. CCK appears to be encoded by a single-copy gene in the haploid human genome, as revealed by genomic Southern hybridization analysis, suggesting that the same gene is expressed both in gut and brain.
基于脑/肠肽胆囊收缩素（CCK）已知氨基酸序列合成了一种合成DNA。该DNA在模糊密码子位置含有脱氧次黄苷，并用作探针直接从人基因组文库中分离CCK基因。对分离出的基因进行核苷酸序列分析表明，人前胰高血糖素由115个氨基酸残基组成，与胃泌素-CCK家族的另一个成员人胃泌素前体有11个氨基酸相同，并且编码区被一个单一的长内含子隔开。基因组Southern杂交分析表明，CCK似乎由单倍体人类基因组中的单拷贝基因编码，这表明同一基因在肠道和大脑中均有表达。
