Wu Jihong, Huo Jiaying, Huang Mingquan, Zhao Mouming, Luo Xuelian, Sun Baoguo
School of Food Science and Engineering, South China University of Technology , Guangzhou, China 510640.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University , Beijing, China 100048.
J Agric Food Chem. 2017 Dec 6;65(48):10495-10504. doi: 10.1021/acs.jafc.7b04815. Epub 2017 Nov 17.
Peptides are rarely reported from Chinese Baijiu (Chinese liquor) because they are often present in very low concentrations in the complex matrix. A tetrapeptide, Ala-Lys-Arg-Ala (AKRA), was recently identified by high-performance liquid chromatography and quadrupole-time-of-flight-mass spectrometry (HPLC-Q-TOF-MS) from sesame flavor-type Baijiu at a concentration of 8.497 ± 0.753 μg/L (P > 0.05), and this tetrapeptide showed preventive effects against 2,2'-azobis(2-methylpropanimidamidine) dihydrochloride (AAPH)-induced oxidative stress in HepG2 cells. The cellular antioxidant activity assay results showed that AKRA protected AAPH-induced oxidative stress in HepG2 cells in a concentration-dependent manner. Pretreatment of the cells for 2 h with AKRA (0.38-1.50 mg/mL) caused strong intracellular reactive oxygen species (ROS) scavenging activities and prevented a decrease in reduced glutathione (GSH) and increases in oxidized glutathione (GSSG) and malondialdehyde (MDA). In addition, AKRA treatment prevented significant decreases in glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) induced by AAPH. Thus, AKRA treatment ameliorated AAPH-induced oxidative stress in HepG2 cells. This study will be important for the design and regulation of functional Baijiu production.
由于肽类物质在复杂基质中的含量通常很低，因此在中国白酒中很少被报道。最近，通过高效液相色谱和四极杆-飞行时间质谱（HPLC-Q-TOF-MS）从芝麻香型白酒中鉴定出一种四肽，即丙氨酸-赖氨酸-精氨酸-丙氨酸（AKRA），其浓度为8.497±0.753μg/L（P>0.05），并且这种四肽对2,2'-偶氮二异丁脒二盐酸盐（AAPH）诱导的HepG2细胞氧化应激具有预防作用。细胞抗氧化活性测定结果表明，AKRA以浓度依赖的方式保护HepG2细胞免受AAPH诱导的氧化应激。用AKRA（0.38-1.50mg/mL）预处理细胞2小时可产生强大的细胞内活性氧（ROS）清除活性，并防止还原型谷胱甘肽（GSH）减少以及氧化型谷胱甘肽（GSSG）和丙二醛（MDA）增加。此外，AKRA处理可防止AAPH诱导的谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）显著降低。因此，AKRA处理改善了AAPH诱导的HepG2细胞氧化应激。这项研究对于功能性白酒生产的设计和调控具有重要意义。
