Department of Biomedical Science and Research Institute for Bioscience and Biotechnology, Hallym University, Chuncheon 24252, Republic of Korea.
Department of Histology and Embryology, Institute of Neuroscience, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
Mol Med Rep. 2018 Jan;17(1):293-299. doi: 10.3892/mmr.2017.7928. Epub 2017 Oct 27.
GABAergic projections terminate on numerous hippocampal interneurons containing calcium binding proteins (CBPs), including calbindin D‑28k (CB), calretinin (CR) and parvalbumin (PV). Memory deficits and expression levels of CB, CR, and PV were examined in the hippocampal subregions following systemic scopolamine (Scop; 1 mg/kg) treatment for 4 weeks in mice. Scop treatment induced significant memory deficits from 1 week after Scop treatment. CB, CR and PV immunoreactivities distributions were in hippocampal subregions [CA1 and CA3 regions, and the dentate gyrus (DG)]. CB immunoreactivity (CB+) was gradually decreased in all subregions until 2 weeks after Scop treatment, and CB+ was decreased to the lowest level in all subregions at 3 and 4 weeks. CR+ in the CA1 region was gradually decreased until 2 weeks and hardly observed at 3 and 4 weeks; in the CA3 region, CR+ was not altered in all subregions at any time. In the DG, CR+ was gradually decreased until 2 weeks and lowest at 3 and 4 weeks. PV+ in the CA1 region was not altered at 1 week, and gradually decreased from 2 weeks. In the CA3 region, PV+ did not change in any subregions at any time. In the DG, PV+ was not altered at 1 week, decreased at 2 weeks, and lowest at 3 and 4 weeks. In brief, Scop significantly decreased CBPs expressions in the hippocampus ≥3 weeks after the treatment although memory deficits had developed at 1 week. Therefore, it is suggested that Scop (1 mg/kg) must be systemically treated for ≥3 weeks to investigate changes in expression levels of CBPs in the hippocampus.
GABA 能神经元投射终止于许多含有钙结合蛋白 (CBPs) 的海马中间神经元，包括钙结合蛋白 D-28k (CB)、钙调蛋白 (CR) 和囊泡相关蛋白 (PV)。在小鼠腹腔注射东莨菪碱 (Scop; 1mg/kg) 4 周后，观察到海马亚区的记忆缺陷和 CB、CR 和 PV 的表达水平。Scop 治疗后 1 周开始出现明显的记忆缺陷。CB、CR 和 PV 免疫反应的分布在海马亚区[CA1 和 CA3 区以及齿状回 (DG)]。CB 免疫反应 (CB+) 在所有亚区逐渐减少，直到 Scop 治疗后 2 周，并且在 3 和 4 周时所有亚区的 CB+均减少至最低水平。CA1 区的 CR+逐渐减少，直到 2 周，3 和 4 周几乎观察不到;在 CA3 区，所有亚区的 CR+在任何时间都没有改变。在 DG 中，CR+逐渐减少，直到 2 周，在 3 和 4 周时最低。CA1 区的 PV+在 1 周时没有改变，从 2 周开始逐渐减少。在 CA3 区，任何亚区在任何时间的 PV+都没有变化。在 DG 中，PV+在 1 周时没有改变，在 2 周时减少，在 3 和 4 周时减少到最低。总之，尽管在 1 周时已经出现了记忆缺陷，但 Scop 在治疗后≥3 周显著降低了海马中的 CBPs 表达。因此，建议 Scop (1mg/kg) 必须系统治疗≥3 周，以研究海马中 CBPs 表达水平的变化。
