de Jong G I, Naber P A, Van der Zee E A, Thompson L T, Disterhoft J F, Luiten P G
Department of Animal Physiology, University of Groningen, Haren, The Netherlands.
Neurobiol Aging. 1996 May-Jun;17(3):459-65. doi: 10.1016/0197-4580(96)00030-9.
Using immunocytochemistry hippocampal levels of the calcium binding proteins calbindin 28K (CB) and parvalbumin (PV) was studied in young (1 month) to very old (60 month) Albino rabbits. Young (3 month) and senescent (30 month) Wistar rats were also examined to compare the distribution and age dependency of PV and CB in both species. The distribution of PV-ir is similar in the rabbit and rat hippocampus. Aging in both species yielded a small loss of PV-ir in axon terminals. The presence of CB-ir interneurons throughout the hippocampus, and the heavy investment of the dentate gyrus (DG) granular cells with CB-ir was also similar in both species. In rabbits, the number of CB-ir interneurons in the CA1, as well as the density of CB-ir in the DG decreased in the first year of life, and did not change between 12-48 months of age. A secondary reduction in the density of CB-ir in the DG was observed at ages beyond 48 months. A similar loss of CB-ir in the DG occurred in the rat. In the CA1, however, the density of CB-ir was similar in young and aged rats. Another remarkable finding was the total absence of CB-ir in CA1 pyramidal neurons of rabbits at any age. Thus, the distribution and age dependency of PV-ir in the hippocampus is similar in both species. The decline of CB-ir in the DG with advancing age is very prominent and may be related to an altered calcium homeostasis in these cells. However, the absence of CB-ir in the CA1 of rabbits makes a causal role for CB in the functional decline of CA1 pyramidal cells during aging unlikely.
利用免疫细胞化学方法，研究了幼年（1个月）至老年（60个月）白化兔海马中钙结合蛋白钙结合蛋白28K（CB）和小白蛋白（PV）的水平。还检测了幼年（3个月）和衰老（30个月）的Wistar大鼠，以比较这两个物种中PV和CB的分布及年龄依赖性。PV免疫反应（PV-ir）在兔和大鼠海马中的分布相似。两个物种的衰老均导致轴突终末中PV-ir略有丢失。CB免疫反应中间神经元在整个海马中的存在，以及齿状回（DG）颗粒细胞中CB免疫反应的大量投入在两个物种中也相似。在兔子中，CA1区CB免疫反应中间神经元的数量以及DG区CB免疫反应的密度在出生后的第一年下降，在12至48个月龄之间没有变化。在48个月龄以后，观察到DG区CB免疫反应密度的二次下降。大鼠的DG区也出现了类似的CB免疫反应丢失。然而，在CA1区，幼年和老年大鼠的CB免疫反应密度相似。另一个显著发现是，在任何年龄的兔CA1锥体细胞中完全没有CB免疫反应。因此，PV免疫反应在海马中的分布和年龄依赖性在两个物种中相似。随着年龄增长，DG区CB免疫反应的下降非常明显，可能与这些细胞中钙稳态的改变有关。然而，兔CA1区缺乏CB免疫反应使得CB在衰老过程中CA1锥体细胞功能衰退中起因果作用的可能性不大。
