College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P.R. China.
J Cell Physiol. 2018 Jun;233(6):4688-4706. doi: 10.1002/jcp.26252. Epub 2018 Jan 15.
Changes in endometrial cell morphology and function are absolutely necessary for successful embryo implantation. In this study, miR-26a was widely expressed in dairy goats, and was found to be regulated by β-estradiol (E2) and progesterone (P4) in endometrial epithelium cells (EECs) as well as stromal cells (ESCs). Furthermore, miR-26a played a role in the regulation of cells proliferation and apoptosis by directly regulating PTEN and indirectly regulating the PI3K/AKT pathway in EECs but not in ESCs of dairy goats in vitro. In addition, miR-26a regulated the expression of osteopontin (OPN), vascular endothelial growth factor (VEGF), Cyclooxygenase-2 (COX-2), and prolactin (PRL) in endometrial cells. Therefore, we could get a conclusion that miR-26a had very complex and diverse functions in the endometrial cells during the development of endometrial receptivity in dairy goats. This study provided an efficient platform for studying the regulatory effect of miR-26a on endometrial cells during the development of endometrial receptivity in dairy goats.
子宫内膜细胞形态和功能的变化对于胚胎着床的成功是绝对必要的。在这项研究中，miR-26a 在奶牛山羊中广泛表达，并被发现受到β-雌二醇（E2）和孕酮（P4）在内膜上皮细胞（EECs）和基质细胞（ESCs）中的调节。此外，miR-26a 通过直接调节 PTEN 以及间接调节 PI3K/AKT 通路在体外调节奶牛山羊 EECs 中的细胞增殖和凋亡，但对 ESCs 没有作用。此外，miR-26a 调节了骨桥蛋白（OPN）、血管内皮生长因子（VEGF）、环氧化酶-2（COX-2）和催乳素（PRL）在子宫内膜细胞中的表达。因此，我们可以得出结论，miR-26a 在奶牛山羊子宫内膜容受性发展过程中对子宫内膜细胞具有非常复杂和多样化的功能。本研究为研究 miR-26a 对奶牛山羊子宫内膜容受性发展过程中子宫内膜细胞的调节作用提供了一个有效的平台。
