College of Animal Science and Technology, Northwest A&F University, Yangling, Shanxi 712100, China.
Genes (Basel). 2020 Sep 7;11(9):1052. doi: 10.3390/genes11091052.
The endometrium undergoes a series of complex changes to form a receptive endometrium (RE) that allows the embryo to be implanted. The inability to establish endometrial receptivity of livestock causes embryo implantation failure and considerable losses to animal husbandry. MicroRNAs (miRNAs) are a class of noncoding RNAs. Studies have found that miRNAs can regulate many critical physiological processes, including the establishment of RE during embryo implantation. miR-184 is highly expressed in the endometrial receptive period of dairy goats. This study aimed to explore the effect of miR-184 on endometrial epithelial cell (EEC) apoptosis and RE establishment. Stanniocalcin2 (STC2) is a direct target of miR-184, and miR-184 decreases the expression of STC2 in dairy goat EECs. miR-184 can activate EECs apoptosis through the RAS/RAF/MEK/ERK pathway. Additionally, miR-184 increases the expression levels of RE marker genes, such as forkhead box M1 (FOXM1) and vascular endothelial growth factor (VEGF). These findings indicate that miR-184 promotes the apoptosis of endometrial epithelial cells in dairy goats by downregulating STC2 via the RAS/RAF/MEK/ERK pathway, and that it may also regulate the establishment of RE in dairy goats.
子宫内膜经历一系列复杂的变化形成接受态子宫内膜（RE），从而允许胚胎着床。家畜中不能建立子宫内膜容受性会导致胚胎着床失败，给畜牧业带来巨大损失。微小 RNA（miRNA）是一类非编码 RNA。研究发现，miRNA 可以调控许多关键的生理过程，包括胚胎着床过程中 RE 的建立。miR-184 在奶牛的子宫内膜接受期高度表达。本研究旨在探讨 miR-184 对子宫内膜上皮细胞（EEC）凋亡和 RE 建立的影响。Stanniocalcin2（STC2）是 miR-184 的直接靶标，miR-184 降低奶牛 EEC 中 STC2 的表达。miR-184 可通过 RAS/RAF/MEK/ERK 通路激活 EEC 凋亡。此外，miR-184 增加了 RE 标记基因（如叉头框蛋白 M1（FOXM1）和血管内皮生长因子（VEGF）的表达水平。这些发现表明，miR-184 通过 RAS/RAF/MEK/ERK 通路下调 STC2 促进奶牛子宫内膜上皮细胞凋亡，并且可能还调控奶牛 RE 的建立。
