Dermatology, The Third People's Hospital of Hangzhou, Hangzhou, China.
Dermatology, Third Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
J Dermatol. 2018 Jan;45(1):31-38. doi: 10.1111/1346-8138.14004. Epub 2017 Nov 8.
To determine the levels and sources of chemokines in the serum and epidermis of vitiligo patients, we examined 80 active patients, 80 stable patients and 40 healthy controls. First, the serum levels of candidate chemokines were measured by Luminex assay, and levels of CCR5, CXCR1 and CXCR3 were measured in peripheral mononuclear cells (PMBC) by flow cytometry. Then, the local epidermis levels of elevated chemokines in vitiligo were tested by Luminex. Finally, the mRNA and protein expression levels of elevated chemokines in HaCaT cells stimulated with interferon (IFN)-γ or tumor necrosis factor (TNF)-α were tested by quantitative real-time polymerase chain reaction and Luminex. The serum levels of CCL5, CXCL8 and CXCL10 in active vitiligo were significantly elevated compared with those in stable vitiligo patients. Furthermore, the levels of CCL3 and CCL4 had weak and positive correlations with the Vitiligo Area Scoring Index. In the peripheral blood of active vitiligo patients, the percentages of CD3 CD8 CCR5 and CD3 CD8 CXCR3 T cells were significantly increased compared with those in stable vitiligo and healthy controls. In the epidermis of lesions, the expression levels of CCL5 and CXCL10 in active vitiligo were significantly increased. In addition, the mRNA and protein levels of CCL5, CXCL8 and CXCL10 were significantly elevated in HaCaT cells after stimulation with TNF-α or IFN-γ. The CCR5/CCL5 and CXCR3/CXCL10 axes may play an important role in the progression and maintenance of vitiligo. Moreover, keratinocytes stimulated with TNF-α and IFN-γ may be a primary source of CCL5 and CXCL10.
为了确定白癜风患者血清和表皮中趋化因子的水平和来源，我们检测了 80 例活动期患者、80 例稳定期患者和 40 例健康对照者。首先，通过 Luminex 分析法检测候选趋化因子的血清水平，并通过流式细胞术检测外周血单个核细胞（PMBC）中 CCR5、CXCR1 和 CXCR3 的水平。然后，通过 Luminex 法检测白癜风患者表皮中升高的趋化因子的水平。最后，通过定量实时聚合酶链反应和 Luminex 法检测干扰素（IFN）-γ或肿瘤坏死因子（TNF）-α刺激的 HaCaT 细胞中升高的趋化因子的 mRNA 和蛋白表达水平。与稳定期白癜风患者相比，活动期白癜风患者血清中 CCL5、CXCL8 和 CXCL10 的水平显著升高。此外，CCL3 和 CCL4 的水平与白癜风面积评分指数呈弱正相关。在活动期白癜风患者的外周血中，CD3+CD8+CCR5 和 CD3+CD8+CXCR3 T 细胞的比例明显高于稳定期白癜风患者和健康对照组。在病变表皮中，活动期白癜风患者的 CCL5 和 CXCL10 表达水平明显升高。此外，在 TNF-α或 IFN-γ刺激后，HaCaT 细胞中 CCL5、CXCL8 和 CXCL10 的 mRNA 和蛋白水平显著升高。CCR5/CCL5 和 CXCR3/CXCL10 轴可能在白癜风的进展和维持中起重要作用。此外，TNF-α和 IFN-γ刺激的角质形成细胞可能是 CCL5 和 CXCL10 的主要来源。
