Fisher-Hoch S P, McCormick J B, Auperin D, Brown B G, Castor M, Perez G, Ruo S, Conaty A, Brammer L, Bauer S
Centers for Disease Control, Atlanta, GA 30333.
Proc Natl Acad Sci U S A. 1989 Jan;86(1):317-21. doi: 10.1073/pnas.86.1.317.
Lassa fever is an acute febrile disease of West Africa, where there are as many as 300,000 infections a year and an estimated 3000 deaths. As control of the rodent host is impracticable at present, the best immediate prospect is vaccination. We tested as potential vaccines in rhesus monkeys a closely related virus, Mopeia virus (two monkeys), and a recombinant vaccinia virus containing the Lassa virus glycoprotein gene, V-LSGPC (four monkeys). Two monkeys vaccinated with the New York Board of Health strain of vaccinia virus as controls died after challenge with Lassa virus. The two monkeys vaccinated with Mopeia virus developed antibodies measurable by radioimmunoprecipitation prior to challenge, and they survived challenge by Lassa virus with minimal physical or physiologic disturbances. However, both showed a transient, low-titer Lassa viremia. Two of the four animals vaccinated with V-LSGPC had antibodies to both Lassa glycoproteins, as determined by radioimmunoprecipitation. All four animals survived a challenge of Lassa virus but experienced a transient febrile illness and moderate physiologic changes following challenge. Virus was recoverable from each of these animals, but at low titer and only during a brief period, as observed for the Mopeia-protected animals. We conclude that V-LSGPC can protect rhesus monkeys against death from Lassa fever.
拉沙热是西非的一种急性发热性疾病，该地区每年有多达30万例感染病例，估计有3000人死亡。由于目前控制啮齿动物宿主不切实际，当前最有希望的方法是接种疫苗。我们在恒河猴身上测试了一种密切相关的病毒——莫佩亚病毒（两只猴子）和一种含有拉沙病毒糖蛋白基因的重组痘苗病毒V-LSGPC（四只猴子）作为潜在疫苗。两只接种纽约市卫生局痘苗病毒株作为对照的猴子在接受拉沙病毒攻击后死亡。两只接种莫佩亚病毒的猴子在攻击前通过放射免疫沉淀法检测到可测量的抗体，它们在接受拉沙病毒攻击后存活下来，身体和生理干扰最小。然而，两者都出现了短暂的低滴度拉沙病毒血症。通过放射免疫沉淀法测定，接种V-LSGPC的四只动物中有两只对两种拉沙糖蛋白都有抗体。所有四只动物在接受拉沙病毒攻击后存活下来，但在攻击后经历了短暂的发热性疾病和中度生理变化。从这些动物中的每一只都能检测到病毒，但滴度较低，且仅在短时间内出现，这与接种莫佩亚病毒保护的动物情况相同。我们得出结论，V-LSGPC可以保护恒河猴免于死于拉沙热。
