Politis M J
Department of Orthopedic Surgery, Shaughnessy Research Centre, Vancouver, British Columbia, Canada.
Plast Reconstr Surg. 1989 Feb;83(2):228-35. doi: 10.1097/00006534-198902000-00004.
Laminin is an extracellular matrix component which can promote neuritic elongation in vitro and has been implicated in the promotion of nerve regeneration in vivo. The present study was undertaken to determine if implantation of Elvax pellets containing exogenous laminin distal to site of lesion could promote regenerative responses in vivo in the adult rat peripheral (sciatic) and central (optic) nerve. In peripheral nerve preparations, Elvax pellets containing laminin or collagen were assessed for their ability to "lure" transected axons into 5-mm-long silicone tubes. In optic nerve studies, laminin pellets were inserted distal to site of nerve crush, and the extent of axonal elongation 2.5 mm to the injury site was assessed. Laminin-containing pellets appeared to support appreciable axonal elongation in both systems. This effect was dose-dependent and not exerted by collagen pellets, substrate-free pellets, or pellets containing irradiated laminin. Collagen IV had some beneficial effect in peripheral, but not central, nerve preparations.
层粘连蛋白是一种细胞外基质成分，它在体外可促进神经突的伸长，并且在体内被认为与促进神经再生有关。本研究旨在确定在成年大鼠外周（坐骨神经）和中枢（视神经）神经损伤部位远端植入含有外源性层粘连蛋白的聚乙烯醋酸乙烯酯小球是否能在体内促进再生反应。在外周神经制备中，评估含有层粘连蛋白或胶原蛋白的聚乙烯醋酸乙烯酯小球“吸引”横断轴突进入5毫米长硅胶管的能力。在视神经研究中，将层粘连蛋白小球插入神经挤压部位远端，并评估损伤部位2.5毫米处轴突伸长的程度。含层粘连蛋白的小球似乎在两个系统中都能支持明显的轴突伸长。这种作用是剂量依赖性的，而胶原蛋白小球、无底物小球或含有经辐射层粘连蛋白的小球则没有这种作用。IV型胶原蛋白在外周神经制备中有一定益处，但在中枢神经制备中则没有。
