Wu Zhihao, Santich Brian H, Liu Hong, Liu Cheng, Cheung Nai-Kong V
Department of Pediatrics, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Gerstner Sloan Kettering Graduate School of Biomedical Sciences, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Methods Mol Biol. 2018;1701:255-269. doi: 10.1007/978-1-4939-7447-4_13.
Antibodies that bind peptide-MHC (pMHC) complex in a manner akin to T-cell receptor (TCR) have not only helped in understanding the mechanism of TCR-pMHC interactions in the context of T-cell biology, but also spurred considerable interest in recent years as potential cancer therapeutics. Traditional methods to generate such antibodies using hybridoma and B-cell sorting technologies are sometimes inadequate, possibly due to the small contribution of peptide to the overall B-cell epitope space on the surface of the pMHC complex (typical peptide MW = 1 kDa versus MHC MW = 45 kDa), and to the multiple efficiency limiting steps inherent in these methods. In this chapter, we describe a phage display approach for the rapid generation of such antibodies with high specificity and affinity.
以类似于T细胞受体（TCR）的方式结合肽 - 主要组织相容性复合体（pMHC）的抗体，不仅有助于在T细胞生物学背景下理解TCR - pMHC相互作用的机制，而且近年来作为潜在的癌症治疗药物也引起了极大的关注。使用杂交瘤和B细胞分选技术产生此类抗体的传统方法有时并不充分，这可能是由于肽对pMHC复合体表面整体B细胞表位空间的贡献较小（典型的肽分子量 = 1 kDa，而MHC分子量 = 45 kDa），以及这些方法中固有的多个效率限制步骤。在本章中，我们描述了一种用于快速产生具有高特异性和亲和力的此类抗体的噬菌体展示方法。
