Beebe T P, Wilson T E, Ogletree D F, Katz J E, Balhorn R, Salmeron M B, Siekhaus W J
Lawrence Livermore National Laboratory, Department of Chemistry, Livermore, CA 94550.
Science. 1989 Jan 20;243(4889):370-2. doi: 10.1126/science.2911747.
Uncoated double-stranded DNA dissolved in a salt solution was deposited on graphite and imaged in air with the scanning tunneling microscope (STM). The resolution was such that the major and minor grooves could be distinguished. The pitch of the helix varied between 27 and 63 angstroms in the images obtained. Thus the STM can be useful for structural studies of a variety of uncoated and isolated biomolecules.
溶解在盐溶液中的未包被双链DNA被沉积在石墨上，并在空气中用扫描隧道显微镜（STM）成像。分辨率足以区分大沟和小沟。在获得的图像中，螺旋间距在27至63埃之间变化。因此，STM可用于各种未包被和分离的生物分子的结构研究。
