噬菌体与免疫系统的相互作用：噬菌体特异性抗体的诱导
Interaction of Bacteriophages with the Immune System: Induction of Bacteriophage-Specific Antibodies.
作者信息
Dąbrowska Krystyna
机构信息
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wrocław, Poland.
出版信息
Methods Mol Biol. 2018;1693:139-150. doi: 10.1007/978-1-4939-7395-8_12.
PMID:29119438
Abstract
In all cases when a bacteriophage makes direct contact with a mammalian organism, it may challenge the mammalian immunological system. Its major consequence is production of antibodies specific to the bacteriophage. Here we present protocols applicable in studies of bacteriophage ability to induce specific antibodies. The protocols have been divided into three parts: purification, immunization, and detection (ELISA).
摘要
在噬菌体与哺乳动物机体直接接触的所有情况下，它都可能挑战哺乳动物的免疫系统。其主要后果是产生针对该噬菌体的特异性抗体。在此，我们介绍适用于研究噬菌体诱导特异性抗体能力的实验方案。这些方案分为三个部分：纯化、免疫和检测（酶联免疫吸附测定）。
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