用于设计噬菌体基因组的合成生物学

Synthetic Biology to Engineer Bacteriophage Genomes.

作者信息

Rita Costa Ana, Milho Catarina, Azeredo Joana, Pires Diana Priscila

机构信息

LIBRO - Laboratory of Research in Biofilms Rosário Oliveira, CEB - Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4700-057, Braga, Portugal.

出版信息

Methods Mol Biol. 2018;1693:285-300. doi: 10.1007/978-1-4939-7395-8_21.

DOI:10.1007/978-1-4939-7395-8_21

PMID:29119447

Abstract

Recent advances in the synthetic biology field have enabled the development of new molecular biology techniques used to build specialized bacteriophages with new functionalities. Bacteriophages have been engineered towards a wide range of applications including pathogen control and detection, targeted drug delivery, or even assembly of new materials.In this chapter, two strategies that have been successfully used to genetically engineer bacteriophage genomes are addressed: a yeast-based platform and bacteriophage recombineering of electroporated DNA.

摘要

合成生物学领域的最新进展推动了新型分子生物学技术的发展，这些技术可用于构建具有新功能的特殊噬菌体。噬菌体已被设计用于广泛的应用，包括病原体控制与检测、靶向药物递送，甚至新材料的组装。在本章中，将介绍两种已成功用于对噬菌体基因组进行基因工程改造的策略：基于酵母的平台和电穿孔DNA介导的噬菌体重组工程。