Moreno-González David, Krulišová Markéta, Gámiz-Gracia Laura, García-Campaña Ana M
Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Granada, Spain.
Charles University, Faculty of Pharmacy in Hradec Králové, Prague, Czech Republic.
Electrophoresis. 2018 Feb;39(4):608-615. doi: 10.1002/elps.201700288. Epub 2017 Nov 29.
A sensitive method using CZE-UV detection has been developed for the determination of five tetracycline antibiotics in human urine samples. To improve the sensitivity of the method, an on-line preconcentration strategy, named field-amplified sample injection, has been developed, based on the electrokinetic injection of the sample, which requires only a 1:100 dilution with sample solvent before injection. Under optimum conditions, sensitivity enhancement factors ranged from 450 to 800 for the studied compounds. The applicability of the proposed method was demonstrated by the determination of these antibiotics in spiked urine samples. The limits of quantification were lower than 0.8 mg/L and the precision (intra- and inter-day), expressed as %RSD was below 14%. Recoveries ranged from 92.1 to 96.7%. Thus, the proposed procedure is a simple, fast and efficient strategy which could be used as therapeutic drug monitoring in human urine samples.
已开发出一种采用毛细管区带电泳-紫外检测的灵敏方法，用于测定人尿液样本中的五种四环素类抗生素。为提高该方法的灵敏度，基于样品的电动进样，开发了一种名为场放大进样的在线预富集策略，进样前仅需用样品溶剂按1:100稀释。在最佳条件下，所研究化合物的灵敏度增强因子在450至800之间。通过测定加标尿液样本中的这些抗生素，证明了该方法的适用性。定量限低于0.8 mg/L，精密度（日内和日间）以相对标准偏差（%RSD）表示低于14%。回收率在92.1%至96.7%之间。因此，所提出的方法是一种简单、快速且高效的策略，可用于人尿液样本中的治疗药物监测。
