Mohammad-Razdari Ayat, Ghasemi-Varnamkhasti Mahdi, Rostami Sajad, Izadi Zahra, Ensafi Ali A, Siadat Maryam
Department of Mechanical Engineering of Biosystems, Shahrekord University, 8818634141 Shahrekord, Islamic Republic of Iran.
Nanotechnology Research Center, Shahrekord University, 8818634141 Shahrekord, Islamic Republic of Iran.
J Food Sci Technol. 2020 Dec;57(12):4697-4706. doi: 10.1007/s13197-020-04506-2. Epub 2020 May 9.
This study dealt with the fabrication of an impedimetric biosensor based on nanomaterial modified with pencil graphite electrode for the detection of tetracycline (TET) in milk samples. For response of the impedimetric aptasensor to be improved, the influence of different parameters (immobilization time of reduced grapheme oxide, time of aptamer, and TET binding, and concentration of aptamer) was optimized. In optimum conditions, the aptasensor provided a concentration range within 1 × 10 - 1 × 10 M and with a limit of detection of 3 × 10 M TET. The proposed impedimetric aptasensor was then used in milk samples analysis, and the acceptable recovery was achieved ranging from 92.8 to 102.1%. According to this study, the combination of an aptamer and electrochemical impedance spectroscopy is a promising method for detection of TET in milk samples with high reproducibility and stability.
本研究涉及基于铅笔石墨电极修饰的纳米材料制备阻抗式生物传感器，用于检测牛奶样品中的四环素（TET）。为了提高阻抗式适配体传感器的响应，对不同参数（还原氧化石墨烯的固定时间、适配体与TET结合时间以及适配体浓度）的影响进行了优化。在最佳条件下，该适配体传感器的检测浓度范围为1×10⁻¹×10⁻⁶ M，TET的检测限为3×10⁻⁷ M。然后将所提出的阻抗式适配体传感器用于牛奶样品分析，回收率在92.8%至102.1%之间，结果令人满意。根据本研究，适配体与电化学阻抗谱相结合是一种检测牛奶样品中TET的有前景的方法，具有高重现性和稳定性。
