Barić Ana, Marković Vinko, Eterović Davor, Bedeković Vladimir, Kontić Mirko, Juretić Kuščić Ljubica, Pešutić Pisac Valdi, Punda Ante
Clinical Department of Nuclear Medicine, Split University Hospital Center, Split
Clinical Department of ENT, Sestre milosrdnice University Hospital Center, Zagreb, Croatia
Acta Clin Croat. 2017 Mar;56(1):15-20. doi: 10.20471/acc.2017.56.01.03.
Th e aim of the study was to determine the influence of RET, p27 and cyclin D1 on regional lymph node metastases in papillary microcarcinoma. The analysis included 70 patients with papillary thyroid microcarcinoma that underwent surgery at Split University Hospital Center between 1999 and 2001. Immunohistochemistry was performed on formalin-fixed, paraffin-embedded tissue by the RET, p27 and cyclin D1 antibodies. Quantification was based on the intensity and distribution of nuclear staining, dividing tumors into those that showed expression (expressors) and those that showed no expression (non-expressors). Univariate analysis using χ²-test and Fisher exact test was performed with the level of statistical significance set at p<0.05. There was no statistically significant difference in the incidence of metastases according to the expression or non-expression of RET mutation (χ²-test: p=0.459; Fisher exact test: p=0.672). Among 25 cases with cyclin D1 expression, 6 had metastases, whereas only 2 of 45 cases with no cyclin D expression had metastases (χ²-test: p=0.014; Fisher exact test: p=0.021), indicating that the expression of cyclin D1 is not crucial for the development of metastases in lymph nodes. In contrast, analysis of p27 expression showed it to be significantly associated with lymph node metastasis because 3 of 45 patients with p27 expression had metastases, indicating a statistically significant correlation between p27 expression and lymph node metastases (χ²-test: p=0.093; Fisher exact test: p=0.124). This study confirmed the importance of the evaluation of RET, p27 and cyclin D1 expression and demonstrated the validity of their application in the assessment of microcarcinoma behavior.
本研究的目的是确定RET、p27和细胞周期蛋白D1对甲状腺微小乳头状癌区域淋巴结转移的影响。分析纳入了1999年至2001年间在斯普利特大学医院中心接受手术的70例甲状腺微小乳头状癌患者。采用RET、p27和细胞周期蛋白D1抗体对福尔马林固定、石蜡包埋的组织进行免疫组织化学检测。定量分析基于细胞核染色的强度和分布，将肿瘤分为有表达的（表达者）和无表达的（非表达者）。使用χ²检验和Fisher精确检验进行单因素分析，设定统计学显著性水平为p<0.05。根据RET突变的表达或不表达，转移发生率无统计学显著差异（χ²检验：p=0.459；Fisher精确检验：p=0.672）。在25例有细胞周期蛋白D1表达的病例中，6例发生转移，而在45例无细胞周期蛋白D表达的病例中只有2例发生转移（χ²检验：p=0.014；Fisher精确检验：p=0.021），这表明细胞周期蛋白D1的表达对淋巴结转移的发生并非至关重要。相反，对p27表达的分析显示其与淋巴结转移显著相关，因为45例有p27表达的患者中有3例发生转移，表明p27表达与淋巴结转移之间存在统计学显著相关性（χ²检验：p=0.093；Fisher精确检验：p=0.124）。本研究证实了评估RET、p27和细胞周期蛋白D1表达的重要性，并证明了它们在评估微小癌行为中的应用价值。
