钩端螺旋体：自然源中致病物种的分子检测
Leptospira: Molecular Detection of Pathogenic Species in Natural Sources.
作者信息
Beigel Brittney, Verma Ashutosh
机构信息
College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee.
Center for Infectious, Zoonotic and Vector-borne Diseases, College of Veterinary Medicine, Lincoln Memorial University, Harrogate, Tennessee.
出版信息
Curr Protoc Microbiol. 2017 Nov 9;47:12E.6.1-12E.6.8. doi: 10.1002/cpmc.43.
This protocol describes a method for the rapid detection of leptospiral DNA in environmental water. In summary, the DNA is extracted from water samples and tested in a TaqMan-based real-time quantitative polymerase chain reaction (qPCR) for the presence of lipl32, a gene that is present only in pathogenic Leptospira spp. The gene target used in this assay is important in that it only detects pathogenic leptospires and not the saprophytic leptospires that may be present in environmental samples. © 2017 by John Wiley & Sons, Inc.
本方案描述了一种快速检测环境水样中钩端螺旋体DNA的方法。简而言之，从水样中提取DNA，并在基于TaqMan的实时定量聚合酶链反应（qPCR）中检测lipl32基因的存在，该基因仅存在于致病性钩端螺旋体属中。本检测中使用的基因靶点很重要，因为它只检测致病性钩端螺旋体，而不检测环境样本中可能存在的腐生性钩端螺旋体。© 2017约翰威立国际出版公司。
Zotero 插件