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通过晶状体蛋白成分分析评估兔眼原位超声处理的效果。

Effects of ultrasonication of the rabbit lens in situ as evaluated by analysis of crystallin composition.

作者信息

Cuthbert J, Phillips C I, Clayton R M, Clarkson D M

出版信息

Trans Ophthalmol Soc U K (1962). 1978;98(4):494-6.

PMID:291212
Abstract

Ultrasonication and aspiration is a recently developed method of lens extraction. We have attempted todetermine whether extraction by this procedure is total or partial and, if so, whether certain regions or components are preferentially removed, and also whether the contents of the lens are physico-chemically modified by the process of extraction. The water-soluble and urea-soluble protein fractions from the ultrasonicated lenses and the contra-lateral intact lenses of six rabbits were analysed by isoelectric focusing. Differences are found in the crystallin content which indicate that extraction is frequently non-representative and that beta- and gamma- crystallins are modified by the process.

摘要

超声乳化吸出术是一种最近开发的晶状体摘除方法。我们试图确定通过该手术进行的摘除是完全的还是部分的,如果是部分摘除,某些区域或成分是否被优先去除,以及晶状体内容物在摘除过程中是否发生了物理化学变化。通过等电聚焦分析了六只兔子经超声处理的晶状体和对侧完整晶状体中的水溶性和尿素溶性蛋白质组分。发现晶状体蛋白含量存在差异,这表明摘除常常不具有代表性,并且β-和γ-晶状体蛋白在该过程中发生了变化。

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Trans Ophthalmol Soc U K (1962). 1978;98(4):494-6.
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