采用离子排斥和离子交换二维色谱系统测定人尿液样本中的γ-羟基丁酸。

Determination of γ-hydroxybutyrate in human urine samples by ion exclusion and ion exchange two-dimensional chromatography system.

作者信息

Liu Junwei, Deng Zhifen, Zhu Zuoyi, Wang Yong, Wang Guoqing, Sun Yu-An, Zhu Yan

机构信息

Department of Applied Chemistry, Zhengzhou University of Light Industry, Zhengzhou 450002, China.

College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China.

出版信息

J Chromatogr A. 2017 Dec 15;1528:35-40. doi: 10.1016/j.chroma.2017.10.069. Epub 2017 Oct 28.

DOI:10.1016/j.chroma.2017.10.069

PMID:29122285

Abstract

A two-dimensional ion chromatography system was developed for the determination of γ-hydroxybutyrate (GHB) in human urine samples. Ion exclusion chromatography was used in the first dimensional separation for elimination of urine matrices and detection of GHB above 10mgL, ion exchange chromatography was used in the second dimensional separation via column-switching technique for detection of GHB above 0.08mgL. Under the optimized chromatographic conditions, the ion exclusion and ion exchange chromatography separation system exhibited satisfactory repeatability (RSD<3.1%, n=6) and good linearity in the range of 50-1000mgL and 0.5-100mgL, respectively. By this method, concentrations of GHB in the selected human urine samples were detected in the range of 0-1.57mgL. The urine sample containing 0.89mgL GHB was selected to evaluate the accuracy; the spiked recoveries of GHB were 95.9-102.8%. The results showed that the two-dimensional ion chromatography system was convenient and practical for the determination of GHB in human urine samples.

摘要

建立了一种二维离子色谱系统用于测定人尿液样本中的γ-羟基丁酸（GHB）。一维分离采用离子排斥色谱法以消除尿液基质并检测浓度高于10mg/L的GHB，二维分离通过柱切换技术采用离子交换色谱法检测浓度高于0.08mg/L的GHB。在优化的色谱条件下，离子排斥和离子交换色谱分离系统分别在50 - 1000mg/L和0.5 - 100mg/L范围内表现出令人满意的重复性（RSD<3.1%，n = 6）和良好的线性。通过该方法，所选人尿液样本中GHB的浓度在0 - 1.57mg/L范围内被检测到。选取含0.89mg/L GHB的尿液样本评估准确度；GHB的加标回收率为95.9 - 102.8%。结果表明，二维离子色谱系统用于测定人尿液样本中的GHB方便且实用。