Wang Changfu, Xin Ping, Wang Youzhi, Zhou Xuegang, Wei Donghua, Deng Chengjie, Sun Shiqin
College of TCM, Guangdong Pharmaceutical University, No. 280 Outside Loop East Road of Higher Education Mega Center, Panyu District, 510006 Guangzhou, Guangdong Province, China.
College of Pharmacy, Harbin Medical University-Daqing, 163319 Daqing, Heilongjiang Province, China.
Fitoterapia. 2018 Jan;124:152-159. doi: 10.1016/j.fitote.2017.11.004. Epub 2017 Nov 6.
Seven new compounds were isolated from the aerial part of Hedyotis diffusa, including three iridoid glycosides, hedyoiridoidside A - C (1-3), two cerebrosides, hedyocerenoside F (4) and G (5), and two new ceramides, hedyoceramide A (6) and B (7). And six known iridoid glycosides (8-13) were also obtained. Their structures were established by their physico-chemical constants and spectroscopic analysis. The cytotoxicity of all compounds against tumor cell lines of human cervical cancer HeLa, human leukemia HL-60, human lung cancer A459, human hepatoma HepG2, human gastric gland carcinoma BCG-823, human nasopharyngeal cancer CNE-2, human colon cancer HCT15, and human prostate cancer PC-3 were also evaluated in vitro. As a result, new compound 1 exhibited evident cytotoxicity to all tumor cell lines, and the IC values are from 9.5μM to 28.2μM, while new compound 2 exhibited evident cytotoxicity to Hela, HL-60, A459, HepG2, BGC-823, CNE-2, and HCT15, and the IC values are from 15.8μM to 26.2μM. Known compound 11 also exhibited evident cytotoxicity to HL-60, A459, HepG2, BGC-823, CNE-2, and HCT15, and the IC values are from 16.5μM to 40.4μM. New compounds 4-7 and known compounds 12 and 13 showed moderate cytotoxicity to some tumor cell lines.
从白花蛇舌草地上部分分离得到7个新化合物，包括3个环烯醚萜苷，即白花蛇舌草环烯醚萜苷A - C（1 - 3），2个脑苷脂，即白花蛇舌草脑苷脂F（4）和G（5），以及2个新的神经酰胺，即白花蛇舌草神经酰胺A（6）和B（7）。还得到了6个已知的环烯醚萜苷（8 - 13）。通过理化常数和光谱分析确定了它们的结构。还体外评估了所有化合物对人宫颈癌HeLa、人白血病HL - 60、人肺癌A459、人肝癌HepG2、人胃腺癌BCG - 823、人鼻咽癌CNE - 2、人结肠癌HCT15和人前列腺癌PC - 3肿瘤细胞系的细胞毒性。结果，新化合物1对所有肿瘤细胞系均表现出明显的细胞毒性，IC值为9.5μM至28.2μM，而新化合物2对HeLa、HL - 60、A459、HepG2、BGC - 823、CNE - 2和HCT15表现出明显的细胞毒性，IC值为15.8μM至26.2μM。已知化合物11对HL - 60、A459、HepG2、BGC - 823、CNE - 2和HCT15也表现出明显的细胞毒性，IC值为16.5μM至40.4μM。新化合物4 - 7以及已知化合物12和13对某些肿瘤细胞系表现出中等细胞毒性。
