肠杆菌科阴沟肠杆菌 HBY 菌株的基因组草图，该临床分离株同时产生 KPC-2 和 NDM-1 碳青霉烯酶。
Draft genome sequence of Enterobacter cloacae HBY, a ST128 clinical strain co-producing KPC-2 and NDM-1 carbapenemases.
机构信息
Centre of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, 158 Shangtang Road, Hangzhou, Zhejiang 310014, China.
Blood Center of Zhejiang Province, 789 Jianye Road, Hangzhou, Zhejiang 310052, China.
出版信息
J Glob Antimicrob Resist. 2018 Mar;12:1-2. doi: 10.1016/j.jgar.2017.10.022. Epub 2017 Nov 6.
OBJECTIVES
Enterobacter cloacae is one of the major pathogens responsible for a variety of human infections. Here we report the draft genome sequence of multidrug-resistant E. cloacae strain HBY isolated from a female patient in China.
METHODS
Whole genomic DNA of E. cloacae strain HBY was extracted and was sequenced using an Illumina HiSeq™ 2000 platform. The generated sequence reads were assembled using CLC Genomics Workbench. The draft genome was annotated using Rapid Annotations using Subsystems Technology (RAST), and the presence of antimicrobial resistance genes was identified.
RESULTS
The 5799439-bp genome contains various antimicrobial resistance genes conferring resistance to aminoglycosides, β-lactams, fosfomycin, macrolides, sulphonamides and fluoroquinolones. Notably, the strain was identified to carry two main carbapenemase genes (bla and bla).
CONCLUSIONS
The genome sequence reported in this study will provide valuable information to understand antibiotic resistance mechanisms in this strain. It is important to monitor the spread strains of Enterobacter sp. encoding both of these carbapenemase genes.
目的
阴沟肠杆菌是引起多种人类感染的主要病原体之一。本研究报告了从中国一位女性患者中分离出的多药耐药阴沟肠杆菌 HBY 菌株的基因组草图序列。
方法
提取阴沟肠杆菌 HBY 菌株的全基因组 DNA，并使用 Illumina HiSeq™ 2000 平台进行测序。使用 CLC Genomics Workbench 对生成的序列进行组装。使用 Rapid Annotations using Subsystems Technology (RAST) 对基因组草图进行注释，并鉴定了抗生素耐药基因的存在。
结果
该 5799439 碱基对的基因组包含了各种抗生素耐药基因，可赋予对氨基糖苷类、β-内酰胺类、磷霉素、大环内酯类、磺胺类和氟喹诺酮类的耐药性。值得注意的是，该菌株被鉴定携带两种主要的碳青霉烯酶基因（bla 和 bla）。
结论
本研究报告的基因组序列将为了解该菌株的抗生素耐药机制提供有价值的信息。监测同时携带这两种碳青霉烯酶基因的肠杆菌属菌株的传播非常重要。
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