Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, Brazil.
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
J Glob Antimicrob Resist. 2018 Dec;15:290-291. doi: 10.1016/j.jgar.2018.11.009. Epub 2018 Nov 15.
The widespread dissemination of extended-spectrum β-lactamase-producing Enterobacteriaceae has become a major issue in veterinary medicine. However, until now, there has been no report of bacteria with such a phenotype in infected snakes. The aim of this study was to report the first draft genome sequence of an Enterobacter cloacae isolate (SERP1) recovered from a snake with infectious stomatitis.
The whole genome of E. cloacae strain SERP1 was sequenced on an Illumina NextSeq platform and was de novo assembled using CLC NGS Cell v.10. Data analysis was performed using online tools from the Center of Genomic Epidemiology.
The genome size was calculated at 4966856bp, containing a total of 4796 protein-coding sequences. The strain was assigned to sequence type 279 (ST279) and, besides the clinically relevant bla and aac(6')-Ib-cr genes, it also presented resistance genes to β-lactams, aminoglycosides, phenicols, sulphonamides, tetracyclines, trimethoprim, quinolones and fosfomycin.
These data offer novel information regarding multidrug-resistant E. cloacae dissemination in wild animals and might contribute to further comparative genomic analysis.
产超广谱β-内酰胺酶的肠杆菌科在兽医领域的广泛传播已成为一个主要问题。然而，迄今为止，尚未有关于此类表型细菌在感染蛇中的报道。本研究旨在报告从患有传染性口炎的蛇中分离出的阴沟肠杆菌（SERP1）的首个基因组草图序列。
采用 Illumina NextSeq 平台对阴沟肠杆菌 SERP1 进行全基因组测序，并使用 CLC NGS Cell v.10 从头组装。数据分析采用基因组流行病学中心的在线工具进行。
基因组大小计算为 4966856bp，包含 4796 个蛋白质编码序列。该菌株被分配到序列类型 279（ST279），除了临床相关的 bla 和 aac(6')-Ib-cr 基因外，还存在对β-内酰胺类、氨基糖苷类、苯氧青霉素类、磺胺类、四环素类、甲氧苄啶、喹诺酮类和磷霉素的耐药基因。
这些数据提供了关于野生动物中多药耐药阴沟肠杆菌传播的新信息，并可能有助于进一步的比较基因组分析。
