Centro de Investigação de Microrganismos, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil.
Centro de Bacteriologia, Instituto Adolfo Lutz, São Paulo, Brazil.
J Glob Antimicrob Resist. 2017 Sep;10:308-309. doi: 10.1016/j.jgar.2017.07.012. Epub 2017 Jul 27.
Enterobacter cloacae has recently emerged as an important agent of nosocomial infections owing to the dissemination of extended-spectrum β-lactamases and carbapenemases in this species. In this context, a rise in the therapeutic use of aminoglycosides was noticed, followed by the accelerated development of resistance mechanisms. In this study, we report the draft genome sequence of a multidrug-resistant E. cloacae subsp. cloacae strain (Ec2) isolated from an active surveillance culture of a patient with Chagas disease.
Whole-genome sequencing (WGS) was performed using a Nextera XT DNA library and NextSeq platform.
WGS analysis revealed the presence of 5527 coding genes, 62 RNA-encoding genes and 275 pseudogenes. Strain Ec2 belongs to sequence type 395 (ST395) and carries 22 transferable antibiotic resistance genes, comprising eight antimicrobial classes, including the rmtD2 gene conferring high-level aminoglycoside resistance.
This draft genome can be used in comparative genomic analyses with different E. cloacae strains. In addition, it could help at elucidating epidemiological aspects regarding the dissemination of clinically relevant resistance genes.
由于广泛存在于该物种中的扩展谱β-内酰胺酶和碳青霉烯酶，阴沟肠杆菌最近已成为医院感染的重要病原体。在这种情况下，氨基糖苷类药物的治疗用途增加，随之而来的是耐药机制的加速发展。在本研究中，我们报告了从恰加斯病患者的主动监测培养物中分离出的多药耐药阴沟肠杆菌亚种阴沟肠杆菌（Ec2）菌株的基因组序列草图。
使用 Nextera XT DNA 文库和 NextSeq 平台进行全基因组测序（WGS）。
WGS 分析显示，该菌株含有 5527 个编码基因、62 个 RNA 编码基因和 275 个假基因。Ec2 菌株属于序列型 395（ST395），携带 22 个可转移抗生素耐药基因，包括 8 个抗菌药物类别，包括赋予高水平氨基糖苷类耐药性的 rmtD2 基因。
该基因组草图可用于与不同阴沟肠杆菌菌株进行比较基因组分析。此外，它还有助于阐明与临床相关耐药基因传播有关的流行病学方面。
