Winzor D J, Stevens A, Augusteyn R C
Department of Biochemistry, University of Queensland, St. Lucia, Australia.
Arch Biochem Biophys. 1989 Jan;268(1):221-6. doi: 10.1016/0003-9861(89)90583-3.
A simple procedure, based on the precipitin curve and the antibody:antigen ratios of the precipitates, is described for evaluation of the intrinsic association constant (k) governing the interaction between a multivalent antigen and a bivalent antibody. Its application is illustrated with a study of the interaction between alpha-crystallin and an elicited monoclonal antibody, which is shown to exhibit essentially identical affinities (k = 9 X 10(4) M-1) for the alpha m and alpha c forms of the antigen.
抗原比率的简单程序，用于评估控制多价抗原与二价抗体之间相互作用的内在缔合常数（k）。通过对α-晶状体蛋白与一种诱导产生的单克隆抗体之间相互作用的研究来说明其应用，结果表明该抗体对α-晶状体蛋白的αm和αc形式表现出基本相同的亲和力（k = 9×10⁴ M⁻¹）。
