Characterization of and allele frequencies in the Japanese population.

INTRODUCTION

Dementia is one of the major health threats to our aging society, and Alzheimer's disease (AD) is the leading cause. In Japan, ∼15% of the elderly population has dementia. The apolipoprotein E () genotype and a polymorphism (rs10524523) in the translocase of outer mitochondrial membrane 40 () gene have been associated with the age of onset of AD. However, differences in allele frequencies of these markers in different ethnic populations are not well known.

METHODS

Whole blood samples were collected from 300 Japanese subjects, and genomic DNA was extracted to determine alleles and rs10524523 genotypes.

RESULTS

Our results indicated that the ε3-'523 short haplotype is less frequent in Japanese subjects than in Caucasians, whereas the ε3-'523 long and ε3-'523 very long haplotypes are more frequent in Japanese subjects than in Caucasians. We also showed that the ε4-'523 short haplotype, which was noted to be frequently observed in African Americans, was also found in the Japanese population, although it is extremely rare in the Caucasian population.

DISCUSSION

A biomarker risk assignment algorithm, using a combination of '523 genotype, and age, has been developed to assign near-term risk for developing the onset of mild cognitive impairment due to AD and is being used as an enrichment tool in an ongoing delay-of-onset clinical trial. Understanding the characterization of and allele frequencies in the Japanese population is the first step in developing a risk algorithm for AD research and clinical applications for AD prevention in Japan.