Aston Institute of Material Research, Aston University, Birmingham B4 7ET, UK.
Lab Chip. 2017 Dec 5;17(24):4225-4230. doi: 10.1039/c7lc00812k.
This paper describes the design, development and successful use of an on-chip goniometer for room-temperature macromolecular crystallography via acoustically induced rotations. We present for the first time a low cost, rate-tunable, acoustic actuator for gradual in-fluid sample reorientation about varying axes and its utilisation for protein structure determination on a synchrotron beamline. The device enables the efficient collection of diffraction data via a rotation method from a sample within a surface confined droplet. This method facilitates efficient macromolecular structural data acquisition in fluid environments for dynamical studies.
本文描述了一种用于室温下大分子晶体学的片上测角仪的设计、开发和成功应用，该测角仪通过声诱导旋转实现。我们首次提出了一种低成本、可调速率的声学驱动器，用于在不同轴线上逐渐对液体中的样品进行重新定向，并将其用于同步辐射光束线上的蛋白质结构测定。该装置可通过在表面限制液滴内的样品进行旋转方法来高效收集衍射数据。该方法便于在流体环境中进行动态研究，从而高效获取大分子结构数据。
