Agaeva E V, Petrov V N, Konoplyannikov A G, Popovkina O E, Lepekhina L A, Sayapina E V, Semenkova I V
A. F. Tsyb Medical Radiology Center, Affiliated Department of National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Obninsk, Russia.
Bull Exp Biol Med. 2017 Nov;164(1):80-84. doi: 10.1007/s10517-017-3929-1. Epub 2017 Nov 9.
In vivo modifying effects of bone marrow mesenchymal stromal cells of humans and laboratory mice on ROS production by mouse blood mononuclears are studied by luminol-dependent zymosan-induced chemiluminescence after syngeneic and xenogeneic transplantation into systemic blood flow. The chemiluminescent activity of mouse blood mononuclears has increased early (1 day) after syngeneic (mouse mesenchymal stromal cells) and xenogeneic (human mesenchymal stromal cells) transplantation. Later, 7-21 days after syngeneic and xenogeneic transplantation, the chemiluminescent activity of mouse mononuclears is suppressed. The probable mechanisms of involvement of the transplanted mesenchymal stromal cells in reprogramming of the blood mononuclear phagocytes from proinflammatory (M1) to anti-inflammatory (M2) phenotype under conditions of their in vivo interactions are discussed; a frequent manifestation of this reprogramming is transition of the phase of activation into inhibition of ROS-producing activity of macrophages.
通过鲁米诺依赖的酵母聚糖诱导的化学发光法，研究了人类和实验小鼠的骨髓间充质基质细胞在同基因和异基因移植入全身血流后对小鼠血液单核细胞产生活性氧的体内修饰作用。在同基因（小鼠间充质基质细胞）和异基因（人类间充质基质细胞）移植后早期（1天），小鼠血液单核细胞的化学发光活性增加。之后，在同基因和异基因移植后7 - 21天，小鼠单核细胞的化学发光活性受到抑制。讨论了移植的间充质基质细胞在体内相互作用条件下参与将血液单核吞噬细胞从促炎（M1）表型重编程为抗炎（M2）表型的可能机制；这种重编程的一个常见表现是巨噬细胞产生活性氧的活性从激活阶段转变为抑制阶段。
