Tsyb A F, Petrov V N, Konoplyannikov A G, Saypina E V, Lepechina L A, Kalsina S Sh, Semenkova I V, Agaeva E V
Medical Radiological Research Center, Russian Academy of Medical Sciences, Obninsk, Russia.
Bull Exp Biol Med. 2008 Jul;146(1):158-64. doi: 10.1007/s10517-008-0238-8.
In vitro chemiluminescent test showed that human bone marrow mesenchymal stem cells and conditioned media dose-dependently inhibit production of reactive oxygen species by macrophages: 50% inhibition of chemiluminescence (compared to biocontrol) was observed at 1:1 mesenchymal stem cell/macrophage ratio or after addition of 20-25% conditioned media to the incubation medium. The observed mechanism of inhibition of production of reactive oxygen forms can play an essential role in the formation of local immunosuppressive microenvironment in the organism after allogenic transplantation of mesenchymal stem cells.
体外化学发光试验表明，人骨髓间充质干细胞和条件培养基可剂量依赖性地抑制巨噬细胞产生活性氧：在间充质干细胞/巨噬细胞比例为1:1或向孵育培养基中添加20 - 25%的条件培养基后，观察到化学发光抑制50%（与生物对照相比）。所观察到的活性氧生成抑制机制可能在间充质干细胞同种异体移植后机体局部免疫抑制微环境的形成中起重要作用。
