Konovalova E V, Shulga A A, Chumakov S P, Khodarovich Yu M, Woo Eui-Jeon, Deev S M
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Korea.
Bull Exp Biol Med. 2017 Nov;164(1):99-101. doi: 10.1007/s10517-017-3933-5. Epub 2017 Nov 10.
Stable red fluorescing line of human ovarian epithelial cancer cells SK-OV-3ip-red was generated expressing gene coding for protein TurboFP635 (Katushka) fluorescing in the far-red spectrum region with excitation and emission peaks at 588 and 635 nm, respectively. Fluorescence of SK-OV-3ip-red line remained high during long-term cell culturing and after cryogenic freezing. The obtained cell line SK-OV-3ip-red can serve a basis for a model of a scattered tumor with numerous/extended metastases and used both for testing anticancer drugs inhibiting metastasis growth and for non-invasive monitoring of the growth dynamics with high precision.
通过表达编码蛋白TurboFP635（Katushka）的基因，产生了人卵巢上皮癌细胞SK-OV-3ip-red的稳定红色荧光系，该蛋白在远红光谱区域发出荧光，激发峰和发射峰分别位于588nm和635nm。在长期细胞培养和低温冷冻后，SK-OV-3ip-red系的荧光仍保持较高水平。所获得的细胞系SK-OV-3ip-red可作为具有大量/广泛转移的散在肿瘤模型的基础，用于测试抑制转移生长的抗癌药物以及高精度非侵入性监测生长动力学。
