State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai, 200433, China.
Plant J. 2018 Jan;93(1):142-154. doi: 10.1111/tpj.13766.
Protein lysine acetylation (KAC) is a dynamic and reversible post-translational modification that has important biological roles in many organisms. Although KAC has been shown to affect reproductive development and meiosis in yeast and animals, similar studies are largely lacking in flowering plants, especially proteome-scale investigations for particular reproductive stages. Here, we report results from a proteomic investigation to detect the KAC status of the developing rice anthers near the time of meiosis (RAM), providing strong biochemical evidence for roles of many KAC-affected proteins during anther development and meiosis in rice. We identified a total of 1354 KAC sites in 676 proteins. Among these, 421 acetylated proteins with 629 KAC sites are novel, greatly enriching our knowledge on KAC in flowering plants. Gene Ontology enrichment analysis showed chromatin silencing, protein folding, fatty acid biosynthetic process and response to stress to be over-represented. In addition, certain potentially specific KAC motifs in RAM were detected. Importantly, 357 rice meiocyte proteins were acetylated; and four proteins genetically identified to be important for rice tapetum and pollen development were acetylated on 14 KAC sites in total. Furthermore, 47 putative secretory proteins were detected to exhibit acetylated status in RAM. Moreover, by comparing our lysine acetylome with the RAM phosphoproteome we obtained previously, we proposed a correlation between KAC and phosphorylation as a potential modulatory mechanism in rice RAM. This study provides the first global survey of KAC in plant reproductive development, making a promising starting point for further functional analysis of KAC during rice anther development and meiosis.
蛋白质赖氨酸乙酰化（KAC）是一种动态的、可逆的翻译后修饰，在许多生物体中具有重要的生物学作用。尽管已经表明 KAC 会影响酵母和动物的生殖发育和减数分裂，但在开花植物中，类似的研究在很大程度上仍然缺乏，尤其是在特定生殖阶段的蛋白质组规模研究。在这里，我们报告了一项蛋白质组学研究的结果，该研究检测了减数分裂时（RAM）水稻花药的 KAC 状态，为 KAC 在水稻花药发育和减数分裂中的作用提供了强有力的生化证据。我们总共鉴定了 676 种蛋白质中的 1354 个 KAC 位点。其中，421 个乙酰化蛋白具有 629 个 KAC 位点是新的，极大地丰富了我们对开花植物中 KAC 的认识。GO 富集分析显示染色质沉默、蛋白质折叠、脂肪酸生物合成过程和应激反应过度表达。此外，在 RAM 中检测到某些潜在的特异性 KAC 基序。重要的是，357 个水稻减数分裂细胞蛋白被乙酰化；并且在总共 14 个 KAC 位点上，四个被遗传鉴定为对水稻绒毡层和花粉发育很重要的蛋白质被乙酰化。此外，在 RAM 中检测到 47 个假定的分泌蛋白表现出乙酰化状态。此外，通过比较我们之前获得的 RAM 赖氨酸乙酰基组和磷酸化组，我们提出了 KAC 与磷酸化之间的相关性，作为水稻 RAM 中的一种潜在调节机制。这项研究提供了植物生殖发育中 KAC 的首次全局调查，为进一步分析 KAC 在水稻花药发育和减数分裂中的功能提供了有希望的起点。
