Key Laboratory of Bio-Resource and Eco-Environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China.
Plant Commun. 2021 Nov 24;3(1):100266. doi: 10.1016/j.xplc.2021.100266. eCollection 2022 Jan 10.
Lysine acetylation (LysAc) is a conserved and important post-translational modification (PTM) that plays a key role in plant physiological and metabolic processes. Based on advances in Lys-acetylated protein immunoenrichment and mass-spectrometric technology, LysAc proteomics studies have been performed in many species. Such studies have made substantial contributions to our understanding of plant LysAc, revealing that Lys-acetylated histones and nonhistones are involved in a broad spectrum of plant cellular processes. Here, we present an extensive overview of recent research on plant Lys-acetylproteomes. We provide in-depth insights into the characteristics of plant LysAc modifications and the mechanisms by which LysAc participates in cellular processes and regulates metabolism and physiology during plant growth and development. First, we summarize the characteristics of LysAc, including the properties of Lys-acetylated sites, the motifs that flank Lys-acetylated lysines, and the dynamic alterations in LysAc among different tissues and developmental stages. We also outline a map of Lys-acetylated proteins in the Calvin-Benson cycle and central carbon metabolism-related pathways. We then introduce some examples of the regulation of plant growth, development, and biotic and abiotic stress responses by LysAc. We discuss the interaction between LysAc and N-terminal acetylation and the crosstalk between LysAc and other PTMs, including phosphorylation and succinylation. Finally, we propose recommendations for future studies in the field. We conclude that LysAc of proteins plays an important role in the regulation of the plant life cycle.
赖氨酸乙酰化 (LysAc) 是一种保守且重要的翻译后修饰 (PTM)，在植物生理和代谢过程中起着关键作用。基于赖氨酸乙酰化蛋白免疫富集和质谱技术的进展，许多物种的 LysAc 蛋白质组学研究已经完成。这些研究为我们理解植物 LysAc 做出了重要贡献，揭示了组蛋白和非组蛋白的赖氨酸乙酰化参与了广泛的植物细胞过程。在这里，我们对植物 Lys-乙酰化蛋白质组学的最新研究进行了广泛的综述。我们深入了解了植物 LysAc 修饰的特征，以及 LysAc 参与细胞过程和调节植物生长发育过程中代谢和生理的机制。首先，我们总结了 LysAc 的特征，包括赖氨酸乙酰化位点的性质、赖氨酸乙酰化赖氨酸侧翼的基序，以及不同组织和发育阶段之间 LysAc 的动态变化。我们还概述了卡尔文-本森循环和中心碳代谢相关途径中赖氨酸乙酰化蛋白质的图谱。然后，我们介绍了 LysAc 对植物生长、发育和生物及非生物胁迫反应的一些调控实例。我们讨论了 LysAc 与 N 端乙酰化之间的相互作用以及 LysAc 与其他翻译后修饰（包括磷酸化和琥珀酰化）之间的串扰。最后，我们对该领域的未来研究提出了建议。我们的结论是，蛋白质的 LysAc 在调节植物生命周期中起着重要作用。
