Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Nanhai Road 7, Qingdao, 266071, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China.
Plant J. 2018 Jan;93(1):119-130. doi: 10.1111/tpj.13764. Epub 2017 Dec 6.
Due to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte, is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation. The developmental process of the protonema resumed, progressing to bud assembly and gametophore formation after transfer to freshwater medium. In addition, a transcriptome analysis showed that plant hormone signal transduction pathways were downregulated when protonema was grown in diluted seawater medium. Consistent with the transcriptome results, the protonema failed to respond to the addition of indole-3-acetic acid and 6-benzylaminopurine to the diluted seawater medium. Based on these results, we concluded that diluted seawater medium blocks the differentiation of protonema. This result could provide a novel insight to benefit future protonema production.
由于其具有高效的同源重组能力和异常的进化地位，苔藓植物拟南芥开始在遗传和进化研究中引起更多关注。原丝体是配子体的丝状阶段，在拟南芥原生质体分离中具有重要意义。此外，原丝体广泛应用于遗传工程。然而，原丝体的诱导和状态维持困难限制了其更广泛的应用。在这项工作中，原丝体在稀释海水中培养基中被高效诱导，并且在没有进一步细胞分化的情况下保持丝状状态。原丝体的发育过程恢复，在转移到淡水培养基后，进行芽组装和配子体形成。此外，转录组分析表明，当原丝体在稀释海水中培养基中生长时，植物激素信号转导途径被下调。与转录组结果一致，原丝体无法对稀释海水中培养基中添加吲哚-3-乙酸和 6-苄基氨基嘌呤做出反应。基于这些结果，我们得出结论，稀释海水培养基阻止了原丝体的分化。这一结果为未来原丝体的生产提供了新的见解。
