Plant Biotechnology, Faculty of Biology, University of Freiburg, 79104, Freiburg, Germany.
BIOSS, Centre for Biological Signalling Studies, 79104, Freiburg, Germany.
Plant Cell Rep. 2018 Oct;37(10):1409-1417. doi: 10.1007/s00299-018-2293-6. Epub 2018 May 24.
During three decades the moss Physcomitrella patens has been developed to a superb green cell factory with the first commercial products on the market. In the past three decades the moss P. patens has been developed from an obscure bryophyte to a model organism in basic biology, biotechnology, and synthetic biology. Some of the key features of this system include a wide range of Omics technologies, precise genome-engineering via homologous recombination with yeast-like efficiency, a certified good-manufacturing-practice production in bioreactors, successful upscaling to 500 L wave reactors, excellent homogeneity of protein products, superb product stability from batch-to-batch, and a reliable procedure for cryopreservation of cell lines in a master cell bank. About a dozen human proteins are being produced in P. patens as potential biopharmaceuticals, some of them are not only similar to their animal-produced counterparts, but are real biobetters with superior performance. A moss-made pharmaceutical successfully passed phase 1 clinical trials, a fragrant moss, and a cosmetic moss-product is already on the market, highlighting the economic potential of this synthetic biology chassis. Here, we focus on the features of mosses as versatile cell factories for synthetic biology and their impact on metabolic engineering.
三十年来，苔藓植物拟南芥已被开发成为一个卓越的绿色细胞工厂，其首款商业产品已投放市场。在过去的三十年中，苔藓拟南芥已从一种不为人知的苔藓植物发展成为基础生物学、生物技术和合成生物学的模式生物。该系统的一些关键特征包括广泛的组学技术、通过类似于酵母的同源重组进行精确的基因组工程、在生物反应器中经过认证的良好生产规范生产、成功扩大到 500L 波浪反应器、蛋白质产品的出色均一性、批间的出色稳定性，以及细胞系在主细胞库中进行冷冻保存的可靠程序。大约有十二种人类蛋白正在拟南芥中被生产作为潜在的生物制药，其中一些不仅与动物生产的对应物相似，而且是具有卓越性能的真正生物改良剂。一种由苔藓制成的药物已成功通过了 I 期临床试验，一种芳香苔藓和一种化妆品苔藓产品已经上市，突显了这种合成生物学底盘的经济潜力。在这里，我们重点介绍苔藓作为合成生物学多功能细胞工厂的特征及其对代谢工程的影响。
