Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics & Molecular Imaging Key Laboratory, Collaborative Innovation Center for Biomedical Engineering, College of Life Science and Technology, and ‡Key Laboratory of Biomedical Photonics (HUST), Ministry of Education, Huazhong University of Science and Technology , Wuhan 430074, Hubei, P. R. China.
ACS Appl Mater Interfaces. 2017 Dec 6;9(48):41748-41759. doi: 10.1021/acsami.7b15296. Epub 2017 Nov 20.
Safe multifunctional nanoplatforms that have multiple therapeutic functions integrated with imaging capabilities are highly desired for biomedical applications. In this paper, targeted chemo-photothermal synergistic therapy and photoacoustic/computed tomography imaging of tumors were achieved by one novel multifunctional nanoprobe (GMS/DOX@SLB-FA); it was composed of a gold nanostar core and a doxorubicin (DOX)-loaded mesoporous silica shell (GMS), which was coated with a folic acid (FA)-modified thermosensitively supported lipid bilayer (SLB-FA) as a gatekeeper. The multifunctional probe had perfect dispersion and stability; 2.1 nm mesoporous pores and 208 nm hydration particle sizes were obtained. In vitro studies indicated that the drug-loaded probe had excellent ability to control the release of DOX, with 71.98 ± 2.52% cumulative release after laser irradiation, which was significantly higher than that of unirradiated control group. A survival rate of 72.75 ± 4.37% of HeLa cells at 57.75 μg/mL probe also demonstrated the low cytotoxicity of the targeted probe. Both in vitro and in vivo results showed that the probe could achieve targeted photoacoustic imaging of tumors because of the fact that the FA-modified probe could specifically recognize the overexpressed FA receptors on tumor cells; meanwhile, the probe could also achieve the chemo-photothermal synergistic therapy of tumors through controlling the drug release from mesoporous channels by a near-infrared laser. Therefore, the probe had great potential in the early diagnosis and treatment of cancer.
用于生物医学应用的安全多功能纳米平台，其特点是具有多种治疗功能并集成成像能力，这是人们所急需的。在本文中，通过一种新型多功能纳米探针（GMS/DOX@SLB-FA）实现了肿瘤的靶向化疗-光热协同治疗和光声/计算机断层扫描成像；它由金纳米星核和载多柔比星（DOX）的介孔硅壳（GMS）组成，其表面包覆有叶酸（FA）修饰的热敏支撑脂质双层（SLB-FA）作为门控。多功能探针具有良好的分散性和稳定性；得到了 2.1nm 的介孔孔和 208nm 的水合粒径。体外研究表明，载药探针具有优异的 DOX 控制释放能力，激光照射后累积释放率为 71.98±2.52%，明显高于未照射对照组。探针浓度为 57.75μg/mL 时 HeLa 细胞的存活率为 72.75±4.37%，也证明了靶向探针的低细胞毒性。体外和体内结果均表明，由于 FA 修饰的探针能够特异性识别肿瘤细胞上过表达的 FA 受体，探针能够实现肿瘤的靶向光声成像；同时，探针还可以通过近红外激光控制介孔通道中的药物释放，实现肿瘤的化疗-光热协同治疗。因此，该探针在癌症的早期诊断和治疗中有很大的应用潜力。
