Song Yuan-Yang, Li Cheng, Yang Xiao-Quan, An Jie, Cheng Kai, Xuan Yang, Shi Xue-Meng, Gao Meng-Juan, Song Xian-Lin, Zhao Yuan-Di, Chen Wei
Britton Chance Center for Biomedical Photonics at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Collaborative Innovation Center for Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, Hubei, P. R. China.
J Mater Chem B. 2018 Aug 7;6(29):4808-4820. doi: 10.1039/c8tb00940f. Epub 2018 Jul 10.
The accurate treatment of tumors with the help of multimodality imaging is of great significance. Herein, a novel multifunctional probe combining active targeted fluorescent imaging (FL)/photoacoustic imaging (PA) and chemo-photothermal therapy for tumors has been designed. Targeting molecule folate (FA) modified graphene oxide (GO) was used to coat core-shell silver sulfide@mesoporous silica (QD@Si) while antitumoral doxorubicin (DOX) was loaded in mesoporous channels by electrostatic adhesion, and a delivery system (QD@Si-D/GO-FA) for active targeted dual-mode imaging and synergistic chemo-photothermal for tumors was successfully obtained. Experiments showed the cell survival rate was 76.3 ± 4.6% when the probe concentration reached 0.5 mg mL, and demonstrated that the probe had good biocompatibility. In vivo and in vitro results indicated that this probe could be used for active targeted FL/PA for tumors with overexpressed FA receptors. The temperature of the tumor rose to 63.5 °C under laser irradiation, and the tumor could be killed more effectively after combination with chemotherapy, which was caused by exfoliation of GO from QD@Si-D/GO-FA after irradiation. These results showed that the probe had great potential for application in oncology and is expected to provide evidence for early diagnosis and treatment of tumors.
借助多模态成像技术对肿瘤进行精准治疗具有重要意义。在此，设计了一种新型多功能探针，其结合了主动靶向荧光成像（FL）/光声成像（PA）以及用于肿瘤治疗的化学光热疗法。靶向分子叶酸（FA）修饰的氧化石墨烯（GO）用于包覆核壳结构的硫化银@介孔二氧化硅（QD@Si），同时通过静电吸附将抗肿瘤药物阿霉素（DOX）负载于介孔通道中，成功获得了一种用于肿瘤主动靶向双模成像及协同化学光热治疗的递送系统（QD@Si-D/GO-FA）。实验表明，当探针浓度达到0.5 mg/mL时，细胞存活率为76.3±4.6%，证明该探针具有良好的生物相容性。体内和体外实验结果表明，该探针可用于对过表达FA受体的肿瘤进行主动靶向FL/PA成像。在激光照射下，肿瘤温度升至63.5℃，与化疗联合后肿瘤能更有效地被杀死，这是由于照射后GO从QD@Si-D/GO-FA上脱落所致。这些结果表明该探针在肿瘤学领域具有巨大的应用潜力，有望为肿瘤的早期诊断和治疗提供依据。
