活红细胞中磷-31和水质子弛豫。在尿毒症中的应用。

Phosphorus-31 and water proton relaxation in living erythrocytes. Application to uremia.

作者信息

Monti J P, Gallice P, Murisasco A, Crevat A

机构信息

Laboratoire de Biophysique, Faculté de Pharmacie, Marseille, France.

出版信息

Biochim Biophys Acta. 1989 Feb 9;1010(2):210-7. doi: 10.1016/0167-4889(89)90163-8.

DOI:10.1016/0167-4889(89)90163-8

PMID:2912503

Abstract

We measured the spin-lattice and spin-spin relaxation times (T1 and T2, respectively) and the nuclear Overhauser effect (NOE) of 31P nuclei of 2,3-diphosphoglycerate (2,3-DPG) in living erythrocytes. The relaxation of water protons was also studied. Phosphorus relaxation is pH-dependent due to a modification of the binding of 2,3-DPG to hemoglobin. We compared the results obtained with normal and uremic erythrocytes. In uremic erythrocytes the 31P relaxation rates are increased, but the intraerythrocytic pH variation in uremic erythrocytes cannot itself explain this increase. A possible role of dialysable substances may explain the increased relaxation rate.

摘要

我们测量了活红细胞中2,3-二磷酸甘油酸（2,3-DPG）的31P核的自旋晶格弛豫时间和自旋-自旋弛豫时间（分别为T1和T2）以及核Overhauser效应（NOE）。还研究了水质子的弛豫。由于2,3-DPG与血红蛋白结合的改变，磷弛豫依赖于pH值。我们比较了正常红细胞和尿毒症红细胞的测量结果。在尿毒症红细胞中，31P弛豫率增加，但尿毒症红细胞内的pH变化本身无法解释这种增加。可透析物质的可能作用或许可以解释弛豫率的增加。