红细胞内pH值和2,3 - 二磷酸甘油酸的31P核磁共振测量。

31P NMR measurements of intracellular pH and 2,3-diphosphoglycerate in erythrocytes.

作者信息

Petersen A, Jacobsen J P, Hørder M

出版信息

Scand J Clin Lab Invest. 1987 May;47(3):233-7. doi: 10.1080/00365518709168896.

Abstract

31P nuclear magnetic resonance (NMR) spectroscopic determinations of 2,3-diphosphoglycerate (2,3-DPG) concentrations in erythrocytes have been compared with values obtained by an enzymatic assay. It was found that the mean difference over a concentration range from 2 to 20 mmol 2,3-DPG/1 red blood cells (RBC) was 0.18 +/- 0.39 (SEM) mmol/l RBC (n = 10). 31P NMR spectroscopy was further tested by simultaneous measurements of 2,3-DPG and intracellular pH under conditions where the concentration of 2,3-DPG was varied. Thereby a linear relation between the pH gradient (delta pH) over the erythrocyte membrane and 2,3-DPG concentrations was found. The data fit a Donnan equilibrium model for the pH difference over the erythrocyte membrane. We conclude that 31P NMR spectroscopy is a sensitive and accurate method that makes possible simultaneous and non-invasive measurements of intracellular pH and 2,3-DPG.

摘要

已将通过31P核磁共振（NMR）光谱法测定红细胞中2,3 - 二磷酸甘油酸（2,3 - DPG）浓度的结果与通过酶促测定法获得的值进行了比较。发现在2至20 mmol 2,3 - DPG/1红细胞（RBC）的浓度范围内，平均差异为0.18±0.39（SEM）mmol/l RBC（n = 10）。通过在2,3 - DPG浓度变化的条件下同时测量2,3 - DPG和细胞内pH值，对31P NMR光谱法进行了进一步测试。由此发现红细胞膜上的pH梯度（ΔpH）与2,3 - DPG浓度之间存在线性关系。数据符合红细胞膜上pH差异的唐南平衡模型。我们得出结论，31P NMR光谱法是一种灵敏且准确的方法，能够同时进行细胞内pH值和2,3 - DPG的非侵入性测量。