Institute for Food Toxicology, University of Veterinary Medicine Hannover, Hannover, Germany.
Department of Cancer Studies, Leicester Royal Infirmary, University of Leicester, Leicester, United Kingdom.
Mol Nutr Food Res. 2018 Jan;62(2). doi: 10.1002/mnfr.201700450. Epub 2018 Jan 9.
Evidence suggests that the dietary consumption of plant extracts containing polyphenols might help prevent the onset of cancers of the gastrointestinal tract. In the present study, the chemopreventive and antiproliferative efficacy of a grapevine shoot extract (Vineatrol®30) containing resveratrol and resveratrol oligomers is investigated in vivo and in vitro.
The in vivo study is performed using Apc mice on a high-fat diet, which represents a model of human adenomatous polyposis, while the potential of the extract as well as some of its isolated constituents to inhibit intestinal adenoma cell proliferation in vitro is investigated using APC10.1 cells derived from an Apc mouse. Vineatrol®30 at a low (2.3 mg kg diet) or high dose (476 mg kg diet) reduces the adenoma number in male and adenoma volume in female animals. Furthermore, Vineatrol®30 as well as resveratrol and two resveratrol tetramers compromise the expansion of APC10.1 cells by reducing cell number, inducing cell cycle arrest, cellular senescence, and apoptosis. However, except for the extract, none of the isolated resveratrol oligomers is more efficacious than resveratrol in these cells.
Vineatrol®30 may merit further investigation as a potential dietary gastrointestinal cancer chemopreventive agent in humans.
有证据表明，食用富含多酚的植物提取物可能有助于预防胃肠道癌症的发生。本研究旨在体内和体外研究一种含有白藜芦醇和白藜芦醇低聚物的葡萄嫩枝提取物（Vineatrol®30）的化学预防和抗增殖作用。
体内研究采用高脂肪饮食的 Apc 小鼠进行，该模型模拟人类腺瘤性息肉病，同时研究了提取物及其一些分离成分对来源于 Apc 小鼠的 APC10.1 细胞的肠道腺瘤细胞增殖的抑制作用。Vineatrol®30 低剂量（2.3mg/kg 饮食）或高剂量（476mg/kg 饮食）可降低雄性动物的腺瘤数量和雌性动物的腺瘤体积。此外，Vineatrol®30 以及白藜芦醇和两种白藜芦醇四聚体通过减少细胞数量、诱导细胞周期停滞、细胞衰老和细胞凋亡来限制 APC10.1 细胞的扩增。然而，除了提取物之外，这些细胞中没有一种分离的白藜芦醇低聚物比白藜芦醇更有效。
Vineatrol®30 可能值得进一步研究，作为人类潜在的胃肠道癌症化学预防饮食剂。
