Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Laboratory of Molecular Biology, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA.
Curr Opin Microbiol. 2018 Apr;42:53-61. doi: 10.1016/j.mib.2017.10.014. Epub 2017 Nov 7.
Hfq, an RNA chaperone, promotes the pairing of small RNAs (sRNAs) to target mRNAs, mediating post-transcriptional regulation of mRNA stability and translation. This regulation contributes to bacterial adaptation during stress and pathogenesis. Recent advances in sequencing techniques demonstrate the presence of sRNAs encoded not only in intergenic regions but also from the 3' and 5' UTRs of mRNAs, expanding sRNA regulatory networks. Additional layers of regulation by Hfq and its associated RNAs continue to be found. Newly identified RNA sponges modulate the activity of some sRNAs. A subset of sRNAs are proving to be bifunctional, able to pair with targets and also encoding small ORFs or binding other RNA binding proteins, such as CsrA. In addition, there are accumulating examples of Hfq inhibiting mRNA translation in the absence of sRNAs.
Hfq 是一种 RNA 伴侣，能促进小 RNA（sRNA）与靶 mRNA 的配对，从而调节 mRNA 稳定性和翻译的转录后水平。这种调控有助于细菌在应激和发病过程中的适应。测序技术的最新进展表明，sRNA 不仅存在于基因间区，也存在于 mRNA 的 3' 和 5' UTR 区，这扩展了 sRNA 调控网络。Hfq 及其相关 RNA 的额外调控层不断被发现。新鉴定的 RNA 海绵调节一些 sRNA 的活性。有一部分 sRNA 被证明具有双重功能，既能与靶标配对，又能编码小 ORF 或结合其他 RNA 结合蛋白，如 CsrA。此外，越来越多的例子表明，Hfq 在没有 sRNA 的情况下也能抑制 mRNA 翻译。
