College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 5;192:27-33. doi: 10.1016/j.saa.2017.10.073. Epub 2017 Oct 31.
A simple and selective colorimetric sensor thioglycolic acid capped silver nanoparticles (TGA-AgNPs) was developed for the detection of 6-benzylaminopurine (6-BAP). The synthesized TGA-AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopic (TEM) techniques. The TGA-AgNPs as a sensor for binding 6-BAP through hydrogen-bonding and π-π bonding that causes large conjugate clusters, resulting in a color change from yellow to reddish orange. The surface plasmon resonance (SPR) band of TGA-AgNPs at 397nm is red-shifted to 510nm, which confirms that 6-BAP induces the aggregation of TGA-AgNPs. Under the optimized conditions, a linear relationship between the absorption ratio (A/A) and 6-BAP concentration was found in the range of 4-26μM. The detection limit of 6-BAP was 0.2μM, which is lower than the other analytical techniques. Moreover, the proposed sensor was successfully applied for the detection of 6-BAP in environmental samples with good recoveries. The proposed assay provides a simple and cost-effective method for the analysis of 6-BAP in vegetable and water samples.
一种简单且选择性的比色传感器巯基乙酸（TGA）修饰的银纳米粒子（TGA-AgNPs）被开发用于检测 6-苄基氨基嘌呤（6-BAP）。合成的 TGA-AgNPs 通过紫外可见光谱、动态光散射（DLS）和透射电子显微镜（TEM）技术进行了表征。TGA-AgNPs 传感器通过氢键和π-π 键与 6-BAP 结合，导致大的共轭簇形成，从而导致颜色从黄色变为红橙色。TGA-AgNPs 的表面等离子体共振（SPR）带在 397nm 处红移至 510nm，这证实了 6-BAP 诱导了 TGA-AgNPs 的聚集。在优化条件下，在 4-26μM 的范围内，吸光度比（A/A）与 6-BAP 浓度之间存在线性关系。6-BAP 的检测限为 0.2μM，低于其他分析技术。此外，该传感器还成功地应用于环境样品中 6-BAP 的检测，回收率良好。该方法为蔬菜和水样中 6-BAP 的分析提供了一种简单且经济有效的方法。
