利用巯基乙酸功能化的银纳米粒子比色识别环境样品中的 6-苄基氨基嘌呤。
Colorimetric recognition of 6-benzylaminopurine in environmental samples by using thioglycolic acid functionalized silver nanoparticles.
机构信息
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 5;192:27-33. doi: 10.1016/j.saa.2017.10.073. Epub 2017 Oct 31.
A simple and selective colorimetric sensor thioglycolic acid capped silver nanoparticles (TGA-AgNPs) was developed for the detection of 6-benzylaminopurine (6-BAP). The synthesized TGA-AgNPs were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and transmission electron microscopic (TEM) techniques. The TGA-AgNPs as a sensor for binding 6-BAP through hydrogen-bonding and π-π bonding that causes large conjugate clusters, resulting in a color change from yellow to reddish orange. The surface plasmon resonance (SPR) band of TGA-AgNPs at 397nm is red-shifted to 510nm, which confirms that 6-BAP induces the aggregation of TGA-AgNPs. Under the optimized conditions, a linear relationship between the absorption ratio (A/A) and 6-BAP concentration was found in the range of 4-26μM. The detection limit of 6-BAP was 0.2μM, which is lower than the other analytical techniques. Moreover, the proposed sensor was successfully applied for the detection of 6-BAP in environmental samples with good recoveries. The proposed assay provides a simple and cost-effective method for the analysis of 6-BAP in vegetable and water samples.
一种简单且选择性的比色传感器巯基乙酸（TGA）修饰的银纳米粒子（TGA-AgNPs）被开发用于检测 6-苄基氨基嘌呤（6-BAP）。合成的 TGA-AgNPs 通过紫外可见光谱、动态光散射（DLS）和透射电子显微镜（TEM）技术进行了表征。TGA-AgNPs 传感器通过氢键和π-π 键与 6-BAP 结合，导致大的共轭簇形成，从而导致颜色从黄色变为红橙色。TGA-AgNPs 的表面等离子体共振（SPR）带在 397nm 处红移至 510nm，这证实了 6-BAP 诱导了 TGA-AgNPs 的聚集。在优化条件下，在 4-26μM 的范围内，吸光度比（A/A）与 6-BAP 浓度之间存在线性关系。6-BAP 的检测限为 0.2μM，低于其他分析技术。此外，该传感器还成功地应用于环境样品中 6-BAP 的检测，回收率良好。该方法为蔬菜和水样中 6-BAP 的分析提供了一种简单且经济有效的方法。
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