Ghodake Gajanan, Shinde Surendra, Saratale Rijuta Ganesh, Kadam Avinash, Saratale Ganesh Dattatraya, Kim Dae-Young
Department Biological and Environmental Science, Dongguk University-Seoul, Ilsandong-gu, 10326, Goyang-si, Gyeonggi-do, Republic of Korea.
Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.
Colloids Surf B Biointerfaces. 2019 Nov 1;183:110436. doi: 10.1016/j.colsurfb.2019.110436. Epub 2019 Aug 9.
The presence of hazardous, radioactive, and rare earth metal such as yttrium (Y) in water poses a serious health concern to the public health, thus, exploring novel Y-binding molecules and colorimetric indicators are desired. Chlortetracycline (CTC)-functionalized silver nanoparticles (AgNPs-CTC) were synthesized, purified by centrifugation and then characterized by UV-vis spectroscopy, XPS, XRD, and HR-TEM. Functionalization of AgNPs with CTC molecules enabled the rapid and sensitive detection of trivalent yttrium ion (Y). A decrease in the intensity of the original surface plasmon resonance peak at 420 nm was observed within the fraction of a min, with the simultaneous appearance of a new peak at a longer wavelength (540 nm); thus, a novel colorimetric and ratiometric absorbance probe was achieved. The free-O-containing moieties of CTC on the AgNPs surface coordinate with Y. Thus, CTC molecules led to the bridging of the AgNPs and subsequent aggregation. A good linear relationship (R = 0.933) in the range of 18 to 243 nM for Y was observed, and the limit of detection (LOD) for ratiometric results was approximately 57.7 nM. The AgNPs-CTC sensor exhibited better colorimetric performance in terms of excellent sensitivity, LOD, and rapid formation of the AgNPs-CTC complex towards Y. The Y spiked water samples from different sources and fetal bovine serum suggest that the developed method is practically useful and essentially portable for on-site monitoring. The AgNPs-CTC sensor can be also applied as a common colorimetric indicator for the detection of trace levels of Y and lanthanides.
水中存在诸如钇（Y）等有害、放射性和稀土金属对公众健康构成严重的健康问题，因此，需要探索新型的Y结合分子和比色指示剂。合成了氯四环素（CTC）功能化的银纳米颗粒（AgNPs-CTC），通过离心进行纯化，然后用紫外可见光谱、X射线光电子能谱、X射线衍射和高分辨率透射电子显微镜进行表征。用CTC分子对AgNPs进行功能化能够快速、灵敏地检测三价钇离子（Y）。在几分钟内就观察到420nm处原始表面等离子体共振峰强度降低，同时在更长波长（540nm）处出现一个新峰；因此，实现了一种新型的比色和比率吸收探针。AgNPs表面的CTC的游离含O部分与Y配位。因此，CTC分子导致AgNPs的桥连和随后的聚集。观察到Y在18至243nM范围内具有良好的线性关系（R = 0.933），比率结果的检测限（LOD）约为57.7nM。AgNPs-CTC传感器在对Y的优异灵敏度、LOD以及AgNPs-CTC复合物的快速形成方面表现出更好的比色性能。来自不同来源的加标Y水样和胎牛血清表明，所开发的方法在实际应用中是有用的，并且基本上便于携带用于现场监测。AgNPs-CTC传感器还可以用作检测痕量Y和镧系元素的通用比色指示剂。
