Animal Products Processing Division, Council of Agriculture-Livestock Research Institute, Taiwan.
Department of Microbiology, Immunology and Biopharmaceuticals National Chiayi University, Taiwan.
Poult Sci. 2018 Jan 1;97(1):236-246. doi: 10.3382/ps/pex300.
Bacillus subtilis var. natto N21 (BS) and different lactic acid bacteria were applied to produce two-stage fermented feeds. Broilers were fed these feeds to select the best fermented feed. The selected fermented feed was pelleted and investigated for its effects on growth performance, carcass traits, intestinal microflora, serum biochemical constituents, and apparent ileal nutrient digestibility. Trial 1 involved three hundred thirty-six 1-d-old broilers with equal numbers of each sex, randomly assigned into control, BS + Bacillus coagulans L12 (BBC), BS + Lactobacillus casei (BLC), BS + Lactobacillus acidophilus (BLA), BS + Lactobacillus acidophilus L15 (BLA15), BS + Lactobacillus delbruekckii (BLD), and BS + Lactobacillus reuteri P24 (BLR24) groups with 3 replicates per group. Trial 2 involved two hundred forty 1-d-old broilers with equal numbers of each sex, randomly assigned into control, BBC, and pelleted BS + Bacillus coagulans L12 fermented feed (PBBC) groups with 4 replicates per group. Trial 3 involved sixteen 21-d-old male broilers randomly assigned into control and PBBC groups with 4 replicates per group for a nutrient digestibility trial. The feed conversion ratio (FCR) in the BBC group was better than the control (P < 0.05), and the production efficiency factor (PEF) was the best. However, weight gain (WG), feed intake (FI), and PEF were the lowest in the BLD group (P < 0.05). The WG during 0 to 21 d and 0 to 35 d in the PBBC groups were higher than the control (P < 0.05). The relative weight of the proventriculus + gizzard in the BBC and PBBC groups were higher than the control (P < 0.05). The digestible amino acid content in the PBBC group increased significantly (P < 0.05). Bacillus coagulans L12 is the best lactic acid bacteria for second stage fermentation. PBBC improved broiler growth performance, which may be due to the higher digestible amino acid content, it has the potential to become a commercial feed.
纳豆芽孢杆菌（BS）和不同的乳酸菌被应用于生产两段式发酵饲料。肉鸡用这些饲料进行喂养，以选择最佳的发酵饲料。选择的发酵饲料被制粒，并研究其对生长性能、屠体特性、肠道微生物区系、血清生化成分和表观回肠养分消化率的影响。试验 1 涉及 336 只 1 日龄肉鸡，公母各半，随机分为对照组、BS+凝结芽孢杆菌 L12（BBC）组、BS+干酪乳杆菌（BLC）组、BS+嗜酸乳杆菌（BLA）组、BS+嗜酸乳杆菌 L15（BLA15）组、BS+德氏乳杆菌保加利亚亚种（BLD）组和 BS+罗伊氏乳杆菌 P24（BLR24）组，每组 3 个重复。试验 2 涉及 240 只 1 日龄肉鸡，公母各半，随机分为对照组、BBC 组和制粒的 BS+凝结芽孢杆菌 L12 发酵饲料（PBBC）组，每组 4 个重复。试验 3 涉及 16 只 21 日龄雄性肉鸡，随机分为对照组和 PBBC 组，每组 4 个重复进行养分消化试验。BBC 组的饲料转化率（FCR）优于对照组（P<0.05），生产效率因子（PEF）最佳。然而，BLD 组的体重增重（WG）、饲料摄入量（FI）和 PEF 最低（P<0.05）。PBBC 组 0 至 21 天和 0 至 35 天的体重增重高于对照组（P<0.05）。BBC 和 PBBC 组的前胃+肌胃相对重量高于对照组（P<0.05）。PBBC 组可消化氨基酸含量显著增加（P<0.05）。凝结芽孢杆菌 L12 是第二段发酵的最佳乳酸菌。PBBC 提高了肉鸡的生长性能，这可能是由于可消化氨基酸含量较高，它有潜力成为一种商业饲料。
