Kim Dong Hyeon, Kim Nam Jun, Zhao Rong Jie, Kim Da Hye, Yang Chae Ha, Kim Hee Young, Gwak Young S, Jang Eun Young, Kim Jae Soo, Lee Yun Kyu, Lee Hyun Jong, Lim Sung Chul, Lee Bong Hyo
Department of Acupuncture, Moxibustion and Acupoint, College of Korean Medicine, Daegu Haany University, Daegu, 42158, Republic of Korea.
School of Mental Health, Qiqihar Medical University, Qiqihar, Heilongjiang Province, 161006, China.
Neurosci Lett. 2018 Jan 18;664:38-42. doi: 10.1016/j.neulet.2017.11.008. Epub 2017 Nov 7.
In the previous studies, it has been demonstrated that acupuncture treatment was effective on the suppression of withdrawal signs as well as self-administration behavior induced by morphine. Based on, the present study has investigated whether acupuncture could attenuate the anxiety-like behavior induced by withdrawal from chronic morphine treatment.
MATERIALS & METHODS: Male Sprague-Dawley rats weighing 270-300g were treated saline or morphine hydrochloride (10mg/kg, s.c.) for 2 weeks. Following abstinence of 5days in home cage, rats were subjected to the measurement of anxiety-like behavior in the elevated plus maze. Bicuculline (1mg/kg, i.p.) and SCH 50911 (2mg/kg, i.p.) were used to investigate the possible mechanism of acupuncture effects focusing on the GABA receptors system.
Acupuncture at HT7 increased the time spent in open arms significantly. Also, these effects of acupuncture at HT7 were blocked by GABA receptor antagonist.
Results of the present study suggest that acupuncture at HT7 can attenuate anxiety-like behavior induced by withdrawal from chronic morphine treatment through the mediation of GABA receptor system.
在先前的研究中，已证明针刺治疗对抑制吗啡诱导的戒断症状以及自我给药行为有效。基于此，本研究调查了针刺是否能减轻慢性吗啡治疗戒断所诱导的焦虑样行为。
体重270 - 300g的雄性Sprague-Dawley大鼠，用生理盐水或盐酸吗啡（10mg/kg，皮下注射）处理2周。在饲养笼中禁欲5天后，大鼠接受高架十字迷宫中焦虑样行为的测量。荷包牡丹碱（1mg/kg，腹腔注射）和SCH 50911（2mg/kg，腹腔注射）用于研究针刺作用聚焦于GABA受体系统的可能机制。
针刺HT7显著增加了在开放臂停留的时间。此外，HT7针刺的这些作用被GABA受体拮抗剂阻断。
本研究结果表明，针刺HT7可通过GABA受体系统的介导减轻慢性吗啡治疗戒断所诱导的焦虑样行为。
