Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou 510631, PR China.
Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Key Laboratory of Ecology and Environmental Science in Guangdong Higher Education, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou 510631, PR China.
Fish Shellfish Immunol. 2018 Feb;73:234-244. doi: 10.1016/j.fsi.2017.11.007. Epub 2017 Nov 7.
Growth, plasma biochemical parameters, fish composition, immune parameters, intestinal histology, and expressions of immune-related genes were examined in hybrid grouper (Epinephelus lanceolatus ♂ × Epinephelus fuscoguttatus ♀) that fed respectively six diets containing Panax notoginseng extract (PNE) at 0, 0.5, 1, 2, 4, and 10 g kg after 8 weeks. Results indicated that dietary PNE significantly improved growth, feed efficiency ratio, protein efficiency ratio, and protein deposit rate, and significantly increased crude protein and crude lipid levels of whole body and crude protein level of muscle. Dietary PNE significantly increased plasma total protein, alkaline phosphatase, immunoglobulin, complement 3 and complement 4 contents, but significantly decreased cholesterol, triglyceride, glucose, and low density lipoprotein cholesterol contents. Furthermore, dietary PNE increased villus length and muscle thickness in foregut, midgut, and hindgut, activities of hepatic superoxide dismutase and total antioxidant capacity, and increased the expression levels of immune related genes (IL-10, TGF-β1, TOR, MHC2 and TLR3) in the head kidney and the expression levels of antioxidant genes (CAT and GR) in fish that fed PNE at 0.5-4 g kg. In conclusion, grouper fed high lipid diets supplemented with PNE at 0.5-10 g kg improved growth, feed utilization, blood immune parameters, hepatic antioxidant status, intestine morphology and expression levels of immune related genes in the head kidney.
在 8 周后，分别给杂交石斑鱼（♀♂）投喂含有 0、0.5、1、2、4 和 10 g/kg 田七提取物（PNE）的 6 种饲料，检测其生长、血浆生化参数、鱼体组成、免疫参数、肠道组织学和免疫相关基因的表达。结果表明，日粮 PNE 显著提高了生长率、饲料效率比、蛋白效率比和蛋白沉积率，显著增加了鱼体和肌肉的粗蛋白和粗脂肪水平。日粮 PNE 显著增加了血浆总蛋白、碱性磷酸酶、免疫球蛋白、补体 3 和补体 4 含量，显著降低了胆固醇、甘油三酯、葡萄糖和低密度脂蛋白胆固醇含量。此外，日粮 PNE 增加了前肠、中肠和后肠的肠绒毛长度和肌肉厚度、肝超氧化物歧化酶和总抗氧化能力的活性，以及头肾中免疫相关基因（IL-10、TGF-β1、TOR、MHC2 和 TLR3）和鱼体抗氧化基因（CAT 和 GR）的表达水平。在高脂日粮中添加 PNE（0.5-10 g/kg）可以提高生长率、饲料利用率、血液免疫参数、肝抗氧化状态、肠道形态和头肾中免疫相关基因的表达水平。
