Institute of Modern Aquaculture Science and Engineering, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Institute of Modern Aquaculture Science and Engineering, Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, School of Life Science, South China Normal University, Guangzhou, 510631, PR China.
Fish Shellfish Immunol. 2020 Mar;98:681-690. doi: 10.1016/j.fsi.2019.11.002. Epub 2019 Nov 4.
The study mainly aimed at the effects of dietary Senecio scandens buch-ham extract (SSBE) on the growth performance, body composition, plasma biochemical index, intestinal and liver histology and the expression of antioxidant, apoptosis and inflammatory related genes in hybrid grouper (Epinephelus lanceolatus♂ × Epinephelus fuscoguttatus♀). Basal diets supplemented with SSBE (10:1) 0%, 0.05%, 0.1%, 0.2% and 0.4% were fed hybrid grouper for 8 weeks. The results showed that WGR and SGR were significantly increased in the week 2 and week 4 in Diet 0.05% group (P < 0.05). The total protein, globulin and albumin significantly increased whereas alanine aminotransferase, triglyceride and alkaline phosphate in the plasma were significantly decreased in Diet 0.1% group (P < 0.05). The villi length, width, muscle thickness and the cross-sectional area of intestine were improved in Diet 0.05% and Diet 0.1% group. The expression levels of PPAR-α and CPT-1 in the liver of hybrid grouper were significantly increased following the supplementation of SSBE (P < 0.05). The expression levels of antioxidant related genes (CAT, GPX, GR and Keap1) and anti-inflammatory factor (IL-10) in liver, head kidney and spleen of hybrid grouper decreased significantly (P < 0.05). In addition, diets supplemented with 0.05%-0.1% SSBE had a good liver-protecting effect, but it would have a detrimental effect on hepatocytes when the content exceeds 0.2%. The above results indicated that the suitable additive amount of SSBE in hybrid grouper feed was 0.05%-0.1%.
本研究主要探讨了菊科千里光属植物千里光提取物（SSBE）对杂交石斑鱼（Epinephelus lanceolatus♂×Epinephelus fuscoguttatus♀）生长性能、体组成、血浆生化指标、肠道和肝脏组织学以及抗氧化、凋亡和炎症相关基因表达的影响。基础饲料中分别添加 0%、0.05%、0.1%、0.2%和 0.4%的 SSBE（10:1），连续投喂杂交石斑鱼 8 周。结果显示，第 2 周和第 4 周时，0.05%组的增重率（WGR）和特定生长率（SGR）显著提高（P<0.05）。0.1%组的血浆总蛋白、球蛋白和白蛋白含量显著升高，而丙氨酸氨基转移酶、甘油三酯和碱性磷酸酶含量显著降低（P<0.05）。0.05%和 0.1%组的肠绒毛长度、宽度、肌层厚度和肠横截面积均有所改善。SSBE 补充后，杂交石斑鱼肝组织中过氧化物酶体增殖物激活受体-α（PPAR-α）和肉毒碱棕榈酰基转移酶 1（CPT-1）的表达水平显著升高（P<0.05）。肝、头肾和脾中抗氧化相关基因（CAT、GPX、GR 和 Keap1）和抗炎因子（IL-10）的表达水平显著降低（P<0.05）。此外，当 SSBE 添加量为 0.05%-0.1%时，对肝脏有较好的保护作用，但当添加量超过 0.2%时，会对肝细胞产生不利影响。上述结果表明，SSBE 适宜添加量为杂交石斑鱼饲料的 0.05%-0.1%。
