The Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada; Departments of Medicine and Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, K1H 8M5, Canada.
The Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, K1H 8L6, Canada; Departments of Medicine and Cellular and Molecular Medicine, University of Ottawa, 451 Smyth Road, Ottawa, ON, K1H 8M5, Canada.
Semin Cell Dev Biol. 2017 Dec;72:10-18. doi: 10.1016/j.semcdb.2017.11.010. Epub 2017 Nov 15.
The Myogenic Regulatory Factors (MRFs) Myf5, MyoD, myogenin and MRF4 are members of the basic helix-loop-helix family of transcription factors that control the determination and differentiation of skeletal muscle cells during embryogenesis and postnatal myogenesis. The dynamics of their temporal and spatial expression as well as their biochemical properties have allowed the identification of a precise and hierarchical relationship between the four MRFs. This relationship establishes the myogenic lineage as well as the maintenance of the terminal myogenic phenotype. The application of genome-wide technologies has provided important new information as to how the MRFs function to activate muscle gene expression. Application of combined functional genomics technologies along with single cell lineage tracing strategies will allow a deeper understanding of the mechanisms mediating myogenic determination, cell differentiation and muscle regeneration.
肌生成调节因子（MRFs）Myf5、MyoD、myogenin 和 MRF4 是基本螺旋-环-螺旋家族转录因子的成员，它们在胚胎发生和出生后肌发生过程中控制骨骼肌细胞的决定和分化。它们的时空表达的动态以及它们的生化特性允许在四个 MRF 之间确定一个精确的和分层的关系。这种关系建立了肌生成谱系以及终末肌表型的维持。全基因组技术的应用提供了关于 MRFs 如何激活肌肉基因表达的重要新信息。结合功能基因组学技术和单细胞谱系追踪策略的应用将允许更深入地了解介导肌生成决定、细胞分化和肌肉再生的机制。
